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Species: Homo sapiens
Genetic Insert: 4-hydroxyphenylpyruvate dioxygenase like
Vector Backbone Description: Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34471290
Proper citation: RRID:Addgene_174131 Copy
Genetic Insert: PYL1-sfGFP-HP1A
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34428454
Proper citation: RRID:Addgene_174132 Copy
Species: Mus musculus
Genetic Insert: Nos1ap
Vector Backbone Description: Backbone Size:5339; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34455393
Proper citation: RRID:Addgene_174133 Copy
Species: Mus musculus
Genetic Insert: Nos1ap
Vector Backbone Description: Backbone Size:5339; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34455393
Proper citation: RRID:Addgene_174134 Copy
Species: Mus musculus
Genetic Insert: Nos1ap
Vector Backbone Description: Backbone Size:5339; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34455393
Proper citation: RRID:Addgene_174135 Copy
Species: Synthetic
Genetic Insert: human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
Vector Backbone Description: Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_174137 Copy
Species: Homo sapiens
Genetic Insert: 4-hydroxyphenylpyruvate dioxygenase like
Vector Backbone Description: Vector Backbone:pLVU; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34471290
Proper citation: RRID:Addgene_174128 Copy
Species: Homo sapiens
Genetic Insert: 4-hydroxyphenylpyruvate dioxygenase like
Vector Backbone Description: Vector Backbone:pLenti62; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34471290
Proper citation: RRID:Addgene_174129 Copy
Species: Synthetic
Genetic Insert: eABE
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34732717
Proper citation: RRID:Addgene_174120 Copy
Species: Homo sapiens
Genetic Insert: CLK1
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:7756; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34346309
Proper citation: RRID:Addgene_174088 Copy
Species: Homo sapiens
Genetic Insert: SRRM2
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:7756; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34346309
Proper citation: RRID:Addgene_174089 Copy
Species: Synthetic
Genetic Insert: HypaR-ABE8e
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34732717
Proper citation: RRID:Addgene_174124 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2827; Vector Backbone:pENTR4; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17407 Copy
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Jacks Lab; Backbone Size:9200; Vector Backbone:Puro.Cre; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18308936
Comments: Puro.Cre was initially generated by amplification of PuroR from MSCV.Puro (Clontech) with PuroF (5’-CGCTGCGCCGAATTCATGACCGAGTACAAGCCCACG-3’) and PuroR (5’-TAAGCGGCCGCTCGAGTCAGGCACCGGGCTTGCGGGTC-3’), digestion with EcoRI and NotI and cloning into UbC.Luciferase.PGK.Cre to generate Puro.Cre.
For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).
Proper citation: RRID:Addgene_17408 Copy
Species: Mus musculus
Genetic Insert: tRNA-ser
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33986151
Proper citation: RRID:Addgene_174318 Copy
Species: Aequorea victoria
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pSin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33986151
Proper citation: RRID:Addgene_174307 Copy
Genetic Insert: Renilla luciferase
Vector Backbone Description: Vector Backbone:pGEM_11Zf; Vector Types:in vitro transcription template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34213414
Comments: The absolute length of the poly(A) region may not be accurate. The poly(A) region before HDV ribozyme may not be completely maintained during amplification from bacteria. Single clones need to be screened to verify poly(A) length.
Proper citation: RRID:Addgene_174338 Copy
Vector Backbone Description: Backbone Size:8020; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34270926
Proper citation: RRID:Addgene_174175 Copy
Species: Other
Genetic Insert: MiCy with mitochondria-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174177 Copy
Species: Streptomyces tendae
Genetic Insert: nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Vector Backbone Description: Backbone Size:3822; Vector Backbone:pCm N-term-SMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35101207
Proper citation: RRID:Addgene_174210 Copy
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