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Species: Synthetic
Genetic Insert: Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
Vector Backbone Description: Backbone Size:4086; Vector Backbone:pCS2+; Vector Types:Multi-purpose expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23953120
Comments: Kirchhofer, A. et al. (2010). Modulation of protein properties in living cells using nanobodies. Nat. Struct. Mol. Biol. 17, 133–138.
Tang J.C. et al. (2013). A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. Cell. 2013 Aug 15;154(4):928-39.
Proper citation: RRID:Addgene_50020 Copy
Species: Other
Genetic Insert: CDS no stop codon, mEOS2 photoactivatable fluorescent protein (Lobophyllia hemprichii)
Vector Backbone Description: Vector Backbone:pAGM1276; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50305 Copy
Species: Other
Genetic Insert: CDS, no stop codon, mCherry variant of RFP (Discosoma sp.)
Vector Backbone Description: Vector Backbone:pAGM1276; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50304 Copy
Species: Other
Genetic Insert: signal peptide, NbCRT calreticulin (Nicotiana benthamiana)
Vector Backbone Description: Vector Backbone:PICH41258; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50307 Copy
Species: Other
Genetic Insert: promoter (0.4 kb), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50268 Copy
Species: Other
Genetic Insert: N terminal Myc tag (4x Myc)
Vector Backbone Description: Vector Backbone:pAGM1276; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50301 Copy
Species: Reptilian orthoreovirus
Genetic Insert: p14FAST
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2549; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25478005
Comments: When p14FAST is expressed in mammalian cells, fusion takes place between expressing cells and any other neighbouring cells that are in contact (even with non-expressing cells of a different cell type). Potential users should note that this implies a risk that cells carrying the fusion gene might fuse with cells of living humans, and potentially carry dangerous genetic material such as oncogenes and viruses into them (even basic cells lines carry mutations making them proliferative and immortal). Appropriate precautions should therefore be taken when handling the cultures.
Proper citation: RRID:Addgene_50025 Copy
Species: Other
Genetic Insert: N terminal HA tag (6x Human influenza hemagglutinin)
Vector Backbone Description: Vector Backbone:pAGM1276; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50300 Copy
Species: Other
Genetic Insert: CDS no stop codon, GFP (A. victoria)
Vector Backbone Description: Vector Backbone:pAGM1276; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50303 Copy
Species: Other
Genetic Insert: C terminal HA tag (6x Human influenza hemagglutinin)
Vector Backbone Description: Vector Backbone:PAGM1301; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50309 Copy
Species: Streptococcus pyogenes
Genetic Insert: SrtA(strep)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22348280
Proper citation: RRID:Addgene_50033 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter + 5' UTR, RbcS2B (AT5g38420, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50275 Copy
Species: Streptococcus pyogenes
Genetic Insert: HA-SrtA(strep)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22348280
Proper citation: RRID:Addgene_50032 Copy
Species: Other
Genetic Insert: promoter, act2 (AT3G18780, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50274 Copy
Species: Other
Genetic Insert: Promoter + 5'UTR, ocs, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50273 Copy
Species: Other
Genetic Insert: promoter, cab2 (AT1g29930, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50279 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter + 5' UTR, LHB1B1 (AT2g34430, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50278 Copy
Species: Other
Genetic Insert: CDS, beta-glucuronidase (E. coli) with two introns,
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50327 Copy
Species: Other
Genetic Insert: CDS, luciferase (P. pyralis)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50326 Copy
Species: Other
Genetic Insert: CDS, mCherry variant of RFP (Discosoma sp.)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50323 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
