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Species: Synthetic
Genetic Insert: ccdB cassette
Vector Backbone Description: Backbone Marker:Yingfeng An; Vector Backbone:pANY4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Plasmid containing T7 promoter and pR18/pL18 in the complementary DNA strands for IPTG-induced or temperature-induced protein expression.
Proper citation: RRID:Addgene_164897 Copy
Species: Caenorhabditis elegans
Genetic Insert: nCre
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164895 Copy
Species: Homo sapiens
Genetic Insert: Small Ubiquitin Like Modifier 1
Vector Backbone Description: Backbone Size:7713; Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25805818
Proper citation: RRID:Addgene_164939 Copy
Species: Homo sapiens
Genetic Insert: Small Ubiquitin Like Modifier 2
Vector Backbone Description: Backbone Size:6325; Vector Backbone:MSCVpuro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25805818
Proper citation: RRID:Addgene_164938 Copy
Species: Synthetic
Genetic Insert: Enhanced green fluorescent protein
Vector Backbone Description: Backbone Size:12176; Vector Backbone:pInducer-10b; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25093678
Proper citation: RRID:Addgene_164935 Copy
Species: Homo sapiens
Genetic Insert: human UBC9
Vector Backbone Description: Backbone Size:6974; Vector Backbone:pMSCVhyg; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25805818
Proper citation: RRID:Addgene_164936 Copy
Species: Caenorhabditis elegans
Genetic Insert: rpl28-rtTA(Q)
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164886 Copy
Species: Caenorhabditis elegans
Genetic Insert: rtetR-zip
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164889 Copy
Species: Synthetic
Genetic Insert: Enhanced green fluorescent protein
Vector Backbone Description: Backbone Size:7140; Vector Backbone:MSCVpic2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27891214
Proper citation: RRID:Addgene_164922 Copy
Species: Synthetic
Genetic Insert: Enhanced green fluorescent protein
Vector Backbone Description: Backbone Size:6735; Vector Backbone:MSCVpic2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27891214
Proper citation: RRID:Addgene_164923 Copy
Species: Caenorhabditis elegans
Genetic Insert: QFAD mutated
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164887 Copy
Species: Homo sapiens
Genetic Insert: KRAS proto-oncogene, GTPase (KRAS)
Vector Backbone Description: Backbone Size:12168; Vector Backbone:pInducer-10b; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25100204
Proper citation: RRID:Addgene_164926 Copy
Species: Synthetic
Genetic Insert: Enhanced green fluorescent protein
Vector Backbone Description: Backbone Size:12176; Vector Backbone:pInducer-10b; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25093678
Proper citation: RRID:Addgene_164925 Copy
Species: Danio rerio
Genetic Insert: kcnma1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Comments: The DNA sequence was amplified from pooled wild type TAB zebrafish embryos. There is a likely polymorphism, N416D, which is not listed in the current ENSEMBL database. This is possibly due to incomplete zebrafish genome annotation, GRCz11. But this will not impact the result of whole mount in situ hybridization
Proper citation: RRID:Addgene_164951 Copy
Species: Danio rerio
Genetic Insert: kcnj13
Vector Backbone Description: Backbone Size:4179; Vector Backbone:pDestTol2pA2; Vector Types:Tol2 transposon destination vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32546498
Proper citation: RRID:Addgene_164950 Copy
Species: Danio rerio
Genetic Insert: kcnmb3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Proper citation: RRID:Addgene_164955 Copy
Species: Danio rerio
Genetic Insert: kcnn1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Comments: The DNA sequence was amplified from pooled wild-type TAB zebrafish embryos. According to the predicted sequence, XP_005171293.1, there are likely polymorphisms, K185E, H323Y, S599N, which are not listed in the current ENSEMBL database. This is possibly due to incomplete zebrafish genome annotation, GRCz11. In addition, at the 5’ end of the insert, there are 44bps extra forward primer dimer sequences (ATGCGGCATCGGCACGCGGGCCATGCGGCATCGGCACGCGGGCC) before the start codon. But this will not impact the result of the whole mount in situ hybridization.
Proper citation: RRID:Addgene_164956 Copy
Species: Danio rerio
Genetic Insert: kcnmb2a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR Cloning Vectror; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Proper citation: RRID:Addgene_164953 Copy
Species: Danio rerio
Genetic Insert: kcnn3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Proper citation: RRID:Addgene_164959 Copy
Species: Danio rerio
Genetic Insert: kcnn1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33728688
Proper citation: RRID:Addgene_164957 Copy
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