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Species: Caenorhabditis elegans
Genetic Insert: <*iA-1xNLS-GFP::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC292; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19646 Copy
Species: Homo sapiens
Genetic Insert: DNAJB6b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19528 Copy
Species: Homo sapiens
Genetic Insert: DnaJ (Hsp40) homolog, subfamily B, member 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19527 Copy
Species: Caenorhabditis elegans
Genetic Insert:
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19648 Copy
Species: Caenorhabditis elegans
Genetic Insert: Phsp-16.2::FLP::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPD118.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19610 Copy
Species: Caenorhabditis elegans
Genetic Insert: Phsp-16.41::FLP::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPD118.28; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19611 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBluescript SK+; Vector Types:carries FLP recombinase cDNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19615 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)-iA-FLP::let-858(3’)-(Cbr)unc-119(+)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC180; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19618 Copy
Species: Caenorhabditis elegans
Genetic Insert: lip-1 prom in PDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:PDONRP4P1R; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_19592 Copy
Species: Caenorhabditis elegans
Genetic Insert: Plag-2::FLP::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJK590; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19620 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ppro-1<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC93; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19623 Copy
Species: Caenorhabditis elegans
Genetic Insert: Plim-7
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19625 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC183; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19624 Copy
Species: Homo sapiens
Genetic Insert: DNAJB2b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19551 Copy
Species: Mus musculus
Genetic Insert: DnaJ (Hsp40) homolog, subfamily B, member 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19552 Copy
Species: Homo sapiens
Genetic Insert: DnaJ (Hsp40) homolog, subfamily B, member 8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19558 Copy
Species: Caenorhabditis elegans
Genetic Insert: pal-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_19603 Copy
Species: Caenorhabditis elegans
Genetic Insert: fbf-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18166083
Proper citation: RRID:Addgene_19602 Copy
Species: Caenorhabditis elegans
Genetic Insert: spe-11 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_19606 Copy
Species: Homo sapiens
Genetic Insert: BAF170
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10078207
Comments: To make pFastBac1-BAF170, BAF170 sequences were transferred into pFastBac1 using NotI and HindIII. A C-terminal Flag epitope was added by PCR. The product of this PCR reaction was digested with SphI and HindIII, transferred into pFastBac1-BAF170. (kingston #1232)
Proper citation: RRID:Addgene_1955 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
