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Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3::mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2416; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Red mCherry expression in the pharynx driven by the myo-3 promoter. unc-54 utr.
Proper citation: RRID:Addgene_19328 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::mCherry
Vector Backbone Description: Backbone Size:2446; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Use as co-injection marker. Red mCherry fluorescence in the pharynx. Do not use at high concentration (above 5 ng/ul) as it is toxic to animals.
Proper citation: RRID:Addgene_19327 Copy
Species: Caenorhabditis elegans
Genetic Insert: rme-2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19382 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-142 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19379 Copy
Species: Caenorhabditis elegans
Genetic Insert: spe-11 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19378 Copy
Species: Caenorhabditis elegans
Genetic Insert: daz-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19391 Copy
Species: Caenorhabditis elegans
Genetic Insert: fog-2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19390 Copy
Species: Caenorhabditis elegans
Genetic Insert: glp-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19393 Copy
Species: Caenorhabditis elegans
Genetic Insert: lip-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19392 Copy
Species: Caenorhabditis elegans
Genetic Insert: pgl-3 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19386 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding A
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19300 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding B
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: Please note that this plasmid is actually quite toxic to cells. From the Sabatini lab:"it is a dominant negative and therefore inhibits the mTORC1 pathway and thus cells."
From the paper:
"To verify the actions of the Rags in a more physiological setting than that achieved by transient cDNA transfection, we generated HEK-293T cell lines stably expressing Rheb1, RagB, or RagBGTP (attempts to generate lines stably expressing RagBGDP failed)."
Proper citation: RRID:Addgene_19302 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding B
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19301 Copy
Species: Caenorhabditis elegans
Genetic Insert: cep-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19389 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding C
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19306 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding C
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19305 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding D
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19308 Copy
Species: Homo sapiens
Genetic Insert: HSPH1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP110 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene. For this particular plasmid, Addgene has confirmed that the HSPH1 has 3 point mutations: D480G, A528V, and N613S. The Kampinga lab considers these SNPs and their effect on function is not known.
Proper citation: RRID:Addgene_19480 Copy
Species: Homo sapiens
Genetic Insert: HSPA1A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP110 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19483 Copy
Species: Homo sapiens
Genetic Insert: HSPH3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP110 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19482 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
