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Species: Schizosaccharomyces pombe
Genetic Insert: his3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P41nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19340 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi5605 targeting region
Vector Backbone Description: Backbone Size:2334; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Standard Multiple Cloning site vector to generate MosSCI inserts at ttTi5605 location. Contains C.Briggsae unc-119 rescue gene. Also contains genomic regions flanking the ttTi5605 Mos1 insertion. Use C.elegans strain EG4322 to generate insertions.
Proper citation: RRID:Addgene_19330 Copy
Species: Caenorhabditis elegans
Genetic Insert: Phsp-16::Mos1 transposase
Vector Backbone Description: Backbone Size:2530; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: This plasmid was made by Dr. Jean Louis Bessereau and described in the publication: Bessereau JL, Wright A, Williams DC, Schuske K, Davis MW, Jorgensen EM.
Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line.
Nature. 2001 Sep 6;413(6851):70-4.
There is che-1 sequence in the plasmid. It comes from the glh-2 3' UTR fragment in the plasmid. The UTR fragment extends into the 3' region of the che-1 gene, which is tail to tail with glh-2 on the chromosome. This is only the last exon of che-1 and it is not relevant to the function of the construct.
Proper citation: RRID:Addgene_19333 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: his3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P3nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19339 Copy
Species: Flag epitope
Genetic Insert: 3FLAG
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pFA6a-kanMX6-P81nmt1-GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19338 Copy
Species: E. coli
Genetic Insert: hphMX6
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pFA6a-5FLAG-kanMX6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19342 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: his3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P81nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19341 Copy
Species: Streptoalloteichus hindustanus
Genetic Insert: bleMX6
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pFA6a-5FLAG-kanMX6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Comments: There are a few point mutations in drug resistant genes. These mutations are derived from the original drug resistant plasmids we have obtained. However these mutations do not alter drug resistant property of the genes or the functions of our plasmids.
Proper citation: RRID:Addgene_19344 Copy
Species: Streptomyces noursei
Genetic Insert: natMX6
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P81nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Comments: There are a few point mutations in drug resistant genes. These mutations are derived from the original drug resistant plasmids we have obtained. However these mutations do not alter drug resistant property of the genes or the functions of our plasmids.
Proper citation: RRID:Addgene_19347 Copy
Species: E. coli
Genetic Insert: hphMX6
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P41nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19349 Copy
Species: Caenorhabditis elegans
Genetic Insert: mes-2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19397 Copy
Species: Caenorhabditis elegans
Genetic Insert: fbf-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19396 Copy
Species: Caenorhabditis elegans
Genetic Insert: pgl-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19399 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding B
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI
Note from the depositor: This plasmid does not express well. This is because it is a dominant negative and therefore inhibits the mTORC1 pathway and thus cells.
Proper citation: RRID:Addgene_19314 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding D
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI
Proper citation: RRID:Addgene_19317 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding D
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI
Proper citation: RRID:Addgene_19316 Copy
Genetic Insert: none
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19319 Copy
Species: Mus musculus
Genetic Insert: ForminI Exon 9
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16480715
Proper citation: RRID:Addgene_19322 Copy
Species: Mus musculus
Genetic Insert: FmnI Iso1b
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16480715
Proper citation: RRID:Addgene_19323 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3::twk-18(cn110)cDNA
Vector Backbone Description: Backbone Size:2446; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Use as co-injection marker. Negative selection marker expressed in the body wall muscles. Animals (almost) normal at 15C, paralyzed at 25C.
Proper citation: RRID:Addgene_19326 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
