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Species: Homo sapiens
Genetic Insert: ELAVL4
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:5372; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_65757 Copy
Species: Homo sapiens
Genetic Insert: ELAVL4
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:2279; Vector Backbone:pENTR 4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_65754 Copy
Species: Homo sapiens
Genetic Insert: ELAVL3
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:2279; Vector Backbone:pENTR 4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_65753 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BspMI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65769 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BsaI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65768 Copy
Species: Homo sapiens
Genetic Insert: ELAVL4
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:5372; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_65763 Copy
Species: Homo sapiens
Genetic Insert: ELAVL3
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:5372; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_65762 Copy
Species: Homo sapiens
Genetic Insert: eRF3a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4572; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25147240
Proper citation: RRID:Addgene_65811 Copy
Species: Synthetic
Genetic Insert: SpCas9 gRNA backbone, without spacer sequence
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26098369
Comments: Use BsmBI sites to insert sgRNA spacer sequence into the vector. See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details.
This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_65777 Copy
Species: Homo sapiens
Genetic Insert: PABPC1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4572; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25147240
Proper citation: RRID:Addgene_65810 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA backbone, without spacer sequence
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26098369
Comments: Use BsmBI sites to insert sgRNA spacer sequence into the vector. See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details.
UPDATE 5/31/2016: The Joung Lab recommends using BPK2660 (Addgene plasmid # 70709) instead of VVT1 as an SaCas9 guide RNA cloning vector, as guide RNAs cloned into BPK2660 are more effective than those cloned into VVT1. The difference in efficacy is due to VVT1 containing the full length guide RNA (predicted from the natural CRISPR sequence in S. aureus), whereas BPK2660 uses a truncated gRNA that is more effective. For the same oligo/spacer, the rate of mutagenesis is greater using BPK2660. See Kleinstiver et al., 2015 Nat Biotech for additional information on the truncated gRNA plasmid (https://www.ncbi.nlm.nih.gov/pubmed/26524662).
Proper citation: RRID:Addgene_65779 Copy
Species: Synthetic
Genetic Insert: gltA2-udhA guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65819 Copy
Species: Synthetic
Genetic Insert: udhA guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65818 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26098369
Proper citation: RRID:Addgene_65774 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
Vector Backbone Description: Vector Backbone:pCAG-CFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26098369
Proper citation: RRID:Addgene_65776 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26098369
Proper citation: RRID:Addgene_65772 Copy
Species: Synthetic
Genetic Insert: zwf guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65825 Copy
Species: Caenorhabditis elegans
Genetic Insert: NLG-1
Vector Backbone Description: Backbone Marker:C.Bargmann; Backbone Size:3500; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18255029
Proper citation: RRID:Addgene_65827 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA
Vector Backbone Description: Backbone Marker:C.I. Bargmann and S. MaCarroll ; Backbone Size:3500; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18255029
Proper citation: RRID:Addgene_65829 Copy
Species: Synthetic
Genetic Insert: proD guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65820 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
