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Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65589 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65582 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65585 Copy
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pDR196; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862
Proper citation: RRID:Addgene_65518 Copy
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pDR196; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862
Proper citation: RRID:Addgene_65514 Copy
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pDR196; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862
Proper citation: RRID:Addgene_65513 Copy
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pDR196; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862
Proper citation: RRID:Addgene_65515 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65592 Copy
Species: Caenorhabditis elegans
Genetic Insert: Peft-3::gfp::h2b::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044730
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_65631 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APs12
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65597 Copy
Species: Caenorhabditis elegans
Genetic Insert: Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044730
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_65632 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65594 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65593 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65596 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65595 Copy
Species: Synthetic
Genetic Insert: attB-mCherry
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, Mouse Targeting, Bxb1; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26007658
Proper citation: RRID:Addgene_65529 Copy
Species: Synthetic
Genetic Insert: attB-mCherry
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, Mouse Targeting, Bxb1; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26007658
Proper citation: RRID:Addgene_65528 Copy
Species: Hepatitis B virus
Genetic Insert: HBV polymerase protein
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18155120
Proper citation: RRID:Addgene_65520 Copy
Species: Staphylococcus aureus
Genetic Insert: mammalian codon-optimized SaCas9, and SaCas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BsaI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65770 Copy
Species: Homo sapiens
Genetic Insert: histone H2B
Vector Backbone Description: Backbone Marker:Addgene #64308; Backbone Size:5600; Vector Backbone:pONL-C1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25831507
Proper citation: RRID:Addgene_65704 Copy
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