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Species: E. coli
Genetic Insert: polyphosphate kinase
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMMB206; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:9143103
Proper citation: RRID:Addgene_18876 Copy
Species: E.coli
Genetic Insert: exopolyphosphatase
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9143103
Proper citation: RRID:Addgene_18877 Copy
Vector Backbone Description: Backbone Size:6573; Vector Backbone:pYUB1049; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17949993
Comments: This is a Gateway destination vector. It requires a ccdB survival strain of E. coli for propagation of the plasmid, but once a gene of interest has been cloned in (via the LR reaction) it will grow in standard E. coli strains (eg. DH5alpha, BL21).
This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning using E. coli.
Proper citation: RRID:Addgene_18878 Copy
Species: Homo sapiens
Genetic Insert: mAmetrine-DEVD-tdTomato
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4000; Vector Backbone:pEYFP-Nuc; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18425137
Proper citation: RRID:Addgene_18879 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: Channelrhodopsin 2-mCherry
Vector Backbone Description: Backbone Size:5340; Vector Backbone:AAV2; Vector Types:AAV, Cre/Lox, Adeno-Associated Virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18614669
Comments: Note: We now recommend the AAV-FLEX-ChR2tdtomato-rev construct. It shows less clumping relative to ChR2 labeled with mCherry.
These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in Stbl2 cells from Invitrogen. Also, to minimize recombination, cells should be cultured at 30 C.
Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.
Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed. The expected patterns can be calculated from the attached sequence.
Proper citation: RRID:Addgene_18916 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS21
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18842 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YPT6
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18845 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GOS1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18846 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YPT1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18848 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CHC1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18850 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC7
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18851 Copy
Species: E. coli
Genetic Insert: optimized maltose FRET sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3000; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18522753
Comments: W230A mutation, which we introduced to change the affinity.
Proper citation: RRID:Addgene_18853 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18522753
Comments: pGWF1 s a Destination Vector. It carries the lethal ccdB gene (The ccdB gene is replaced with your gene of interest during the LR reaction). The Destination Vectors will only grow on ccdB resistant cells from Invitrogen, Catalog no. A10460.
Proper citation: RRID:Addgene_18856 Copy
Species: Homo sapiens
Genetic Insert: Stromal interaction molecule 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:Gateway destination; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16005298
Proper citation: RRID:Addgene_18858 Copy
Species: Homo sapiens
Genetic Insert: Stromal interaction molecule 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:Gateway destination; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16005298
Proper citation: RRID:Addgene_18859 Copy
Species: Streptomyces phage phiC31
Genetic Insert: phiC31 integrase
Vector Backbone Description: Backbone Size:3016; Vector Backbone:pACYC177; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10801973
Proper citation: RRID:Addgene_18941 Copy
Species: Streptomyces phage phiC31
Genetic Insert: phiC31 integrase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5632; Vector Backbone:pET11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15126397
Proper citation: RRID:Addgene_18942 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia inducible factor 1 alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5433; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12086860
Proper citation: RRID:Addgene_18949 Copy
Species: E. coli
Genetic Insert: arabinose transporter araE under control of Ptac
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMMB207; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11092865
Proper citation: RRID:Addgene_19001 Copy
Species: E. herbicola
Genetic Insert: crtE, crtB
Vector Backbone Description: Backbone Marker:Cunningham et al., 1994; Backbone Size:0; Vector Backbone:pAC-LYC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11676567
Proper citation: RRID:Addgene_19002 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
