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Species: Mus musculus
Genetic Insert: Type II transmembrane serine protease 6
Vector Backbone Description: Backbone Size:6265; Vector Backbone:p3xFLAG-CMV-14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18451267
Proper citation: RRID:Addgene_18789 Copy
Vector Backbone Description: Backbone Size:8718; Vector Backbone:pJG4-5; Vector Types:Yeast Expression, two-hybrid prey vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pJG4-5 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a prey vector expression clone for yeast two-hybrid.
Proper citation: RRID:Addgene_18704 Copy
Vector Backbone Description: Backbone Size:12414; Vector Backbone:pEG202; Vector Types:Yeast Expression, two-hybrid bait vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pEG202 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a bait vector expression clone for yeast two-hybrid.
Proper citation: RRID:Addgene_18705 Copy
Species: Mus musculus
Genetic Insert: Type II transmembrane serine protease 6
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1 (Clontech); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18451267
Proper citation: RRID:Addgene_18792 Copy
Species: Mus musculus
Genetic Insert: Type II transmembrane serine protease 6
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1 (Clontech); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18451267
Proper citation: RRID:Addgene_18793 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sir4
Vector Backbone Description: Backbone Size:7167; Vector Backbone:pOBD2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18802438
Proper citation: RRID:Addgene_18795 Copy
Species: Homo sapiens
Genetic Insert: 8-oxoguanine DNA glycosylase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pMCV/myc/nuc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17018150
Proper citation: RRID:Addgene_18710 Copy
Species: Homo sapiens
Genetic Insert: NEDD8
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9353319
Comments: Note that this is the active form of NEDD8. Pro-NEDD8 (which is inactive) has an additional 5 amino acids at the c-terminus.
Proper citation: RRID:Addgene_18711 Copy
Species: Homo sapiens
Genetic Insert: ubiquitin
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9353319
Comments: This plasmid grows slowly. We encourage requesting scientists to grow for a longer period of time than normal. Please note that this plasmid runs as a dimer (>10kb) or multimer.
Proper citation: RRID:Addgene_18712 Copy
Species: Homo sapiens
Genetic Insert: SENP2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:PFLAG-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20417598
Proper citation: RRID:Addgene_18713 Copy
Species: Homo sapiens
Genetic Insert: SENP3m
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-RGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16608850
Proper citation: RRID:Addgene_18714 Copy
Species: Homo sapiens
Genetic Insert: FLAG-SENP6m
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:PFLAG-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20705237
Proper citation: RRID:Addgene_18716 Copy
Species: Homo sapiens
Genetic Insert: Cdk5
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV-neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8266103
Comments: See scanned map.
Proper citation: RRID:Addgene_1871 Copy
Vector Backbone Description: Backbone Size:6300; Vector Backbone:MSCV IRES Luciferase; Vector Types:Mammalian Expression, Retroviral, Luciferase; Bacterial Resistance:Ampicillin
Comments: To create MSCV/IRES/LUC, the MSCV/IRES/GFP plasmid was amplified up and digested with NcoI and SalI to remove the GFP. Next, the pGL3-Promoter vector from Promega was amplified up and digested with NcoI and SalI to remove luciferase and the Poly(A) signal. Once completed, the 1.7kb luciferase was ligated into MSCV/IRES to give the MSCV/IRES/LUC plasmid.
Proper citation: RRID:Addgene_18760 Copy
Species: Homo sapiens
Genetic Insert: Cdk4
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV-neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8266103
Comments: See scanned map.
Proper citation: RRID:Addgene_1874 Copy
Species: Homo sapiens
Genetic Insert: Cdk4
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV-neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8266103
Comments: See scanned map.
Proper citation: RRID:Addgene_1875 Copy
Species: Homo sapiens
Genetic Insert: Cdk4
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV-neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8266103
Comments: See scanned map.
Proper citation: RRID:Addgene_1876 Copy
Species: Mus musculus
Genetic Insert: eIF4E E103A
Vector Backbone Description: Backbone Size:0; Vector Backbone:MSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18055695
Proper citation: RRID:Addgene_18766 Copy
Species: Mus musculus
Genetic Insert: Dom neg E-cadherin
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL-Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18483246
Comments: Truncated murine E-cadherin cDNA cloned into pWZL blast.
Proper citation: RRID:Addgene_18800 Copy
Species: Homo sapiens
Genetic Insert: E-cadherin shRNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1 hygro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18483246
Comments: siRNA directed against E-cadherin: 5'-AAGATAGGAGTTCTCTGATGC-3'. Sequence from the website of the RNAi consortium at the Broad institute.
Please note that the sequence originally published in the paper was incorrect and the sequence above is the actual shRNA used.
Proper citation: RRID:Addgene_18802 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
