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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
shERK2a-mlpx-puro
 
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RRID:Addgene_65229 shRNA against ERK2 Homo sapiens Ampicillin PMID:23599344 Backbone Size:6533; Vector Backbone:pMLPX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:16:30 0
pYTK076
 
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RRID:Addgene_65183 HIS3 Saccharomyces cerevisiae Chloramphenicol PMID:25871405 Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-01 01:16:38 0
pYTK075
 
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RRID:Addgene_65182 LEU2 Saccharomyces cerevisiae Chloramphenicol PMID:25871405 Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-01 01:16:30 0
pYTK078
 
Resource Report
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1+ mentions
RRID:Addgene_65185 NourseothricinR Synthetic Chloramphenicol PMID:25871405 Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-01 01:16:30 1
pYTK077
 
Resource Report
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1+ mentions
RRID:Addgene_65184 KanamycinR Synthetic Chloramphenicol PMID:25871405 Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-01 01:16:30 2
pYTK074
 
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RRID:Addgene_65181 URA3 Saccharomyces cerevisiae Chloramphenicol PMID:25871405 Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-01 01:16:30 0
pYTK073
 
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RRID:Addgene_65180 ConRE' Synthetic Chloramphenicol PMID:25871405 Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-01 01:16:38 0
pRK5RS-HERCD533
 
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1+ mentions
RRID:Addgene_65224 HERCD533 Homo sapiens Ampicillin PMID:1346334 Insert codes for AA 1-653 (extracellular and transmembrane domains) Note: Amino acid number may be off when compared to current NCBI entry. Please check QC sequence to verify end of coding region. Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin deleted AA 654-1186 2026-08-01 01:16:39 1
pYTK080
 
Resource Report
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1+ mentions
RRID:Addgene_65187 ZeocinR Synthetic Chloramphenicol PMID:25871405 Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-01 01:16:30 1
pDCAF3-IS5
 
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1+ mentions
RRID:Addgene_65220 ndmA Pseudomonas putida strain CBB5 Chloramphenicol and Kanamycin PMID:23654268 The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3. Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100). N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See: Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049. For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC). Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin 2026-08-01 01:16:30 1
pLXSN-Axl
 
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1+ mentions
RRID:Addgene_65222 AXL Homo sapiens Ampicillin PMID:18339872 Backbone Marker:Clontech; Backbone Size:5900; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-01 01:16:30 1
pYTK081
 
Resource Report
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1+ mentions
RRID:Addgene_65188 CEN6/ARS4 Saccharomyces cerevisiae Chloramphenicol PMID:25871405 Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-01 01:16:30 1
pcDNA3-Delta(Pro-MP)ADAM17-HA
 
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RRID:Addgene_65221 Delta(Pro-MP)ADAM17 Homo sapiens Ampicillin PMID:12743035 Mutagenesis creates EcoRV site 57bp downstream of Start ATG. Insert contains several point mutations compared to NM_003183 (see sequence). Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin deleted AA 18-473 2026-08-01 01:16:39 0
pYTK087
 
Resource Report
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RRID:Addgene_65194 LEU2 3' Homology Saccharomyces cerevisiae Chloramphenicol PMID:25871405 Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-01 01:16:30 0
pYTK086
 
Resource Report
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RRID:Addgene_65193 URA3 3' Homology Saccharomyces cerevisiae Chloramphenicol PMID:25871405 Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-01 01:16:30 0
pYTK083
 
Resource Report
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1+ mentions
RRID:Addgene_65190 AmpR-ColE1 Synthetic Ampicillin PMID:25871405 Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:16:30 4
pYTK085
 
Resource Report
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1+ mentions
RRID:Addgene_65192 SpecR-ColE1 Synthetic Spectinomycin PMID:25871405 Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-01 01:16:38 2
pYTK084
 
Resource Report
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RRID:Addgene_65191 KanR-ColE1 Synthetic Kanamycin PMID:25871405 Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:16:30 0
pcDNA3.1 mCherry Gamma 3
 
Resource Report
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RRID:Addgene_65234 mcherry Ampicillin PMID:24988358 Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:16:30 0
shCTL-mlpx-puro
 
Resource Report
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RRID:Addgene_65232 non-coding shRNA Homo sapiens Ampicillin PMID:23599344 Backbone Size:6533; Vector Backbone:pMLPX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:16:30 0

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