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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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shERK2a-mlpx-puro Resource Report Resource Website |
RRID:Addgene_65229 | shRNA against ERK2 | Homo sapiens | Ampicillin | PMID:23599344 | Backbone Size:6533; Vector Backbone:pMLPX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:16:30 | 0 | ||
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pYTK076 Resource Report Resource Website |
RRID:Addgene_65183 | HIS3 | Saccharomyces cerevisiae | Chloramphenicol | PMID:25871405 | Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:16:38 | 0 | |
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pYTK075 Resource Report Resource Website |
RRID:Addgene_65182 | LEU2 | Saccharomyces cerevisiae | Chloramphenicol | PMID:25871405 | Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:16:30 | 0 | |
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pYTK078 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65185 | NourseothricinR | Synthetic | Chloramphenicol | PMID:25871405 | Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:16:30 | 1 | |
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pYTK077 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65184 | KanamycinR | Synthetic | Chloramphenicol | PMID:25871405 | Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:16:30 | 2 | |
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pYTK074 Resource Report Resource Website |
RRID:Addgene_65181 | URA3 | Saccharomyces cerevisiae | Chloramphenicol | PMID:25871405 | Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:16:30 | 0 | |
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pYTK073 Resource Report Resource Website |
RRID:Addgene_65180 | ConRE' | Synthetic | Chloramphenicol | PMID:25871405 | Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:16:38 | 0 | |
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pRK5RS-HERCD533 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65224 | HERCD533 | Homo sapiens | Ampicillin | PMID:1346334 | Insert codes for AA 1-653 (extracellular and transmembrane domains) Note: Amino acid number may be off when compared to current NCBI entry. Please check QC sequence to verify end of coding region. | Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | deleted AA 654-1186 | 2026-08-01 01:16:39 | 1 |
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pYTK080 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65187 | ZeocinR | Synthetic | Chloramphenicol | PMID:25871405 | Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:16:30 | 1 | |
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pDCAF3-IS5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65220 | ndmA | Pseudomonas putida strain CBB5 | Chloramphenicol and Kanamycin | PMID:23654268 | The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3. Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100). N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See: Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049. For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC). Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin | 2026-08-01 01:16:30 | 1 | |
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pLXSN-Axl Resource Report Resource Website 1+ mentions |
RRID:Addgene_65222 | AXL | Homo sapiens | Ampicillin | PMID:18339872 | Backbone Marker:Clontech; Backbone Size:5900; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:16:30 | 1 | ||
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pYTK081 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65188 | CEN6/ARS4 | Saccharomyces cerevisiae | Chloramphenicol | PMID:25871405 | Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:16:30 | 1 | |
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pcDNA3-Delta(Pro-MP)ADAM17-HA Resource Report Resource Website |
RRID:Addgene_65221 | Delta(Pro-MP)ADAM17 | Homo sapiens | Ampicillin | PMID:12743035 | Mutagenesis creates EcoRV site 57bp downstream of Start ATG. Insert contains several point mutations compared to NM_003183 (see sequence). | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | deleted AA 18-473 | 2026-08-01 01:16:39 | 0 |
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pYTK087 Resource Report Resource Website |
RRID:Addgene_65194 | LEU2 3' Homology | Saccharomyces cerevisiae | Chloramphenicol | PMID:25871405 | Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:16:30 | 0 | |
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pYTK086 Resource Report Resource Website |
RRID:Addgene_65193 | URA3 3' Homology | Saccharomyces cerevisiae | Chloramphenicol | PMID:25871405 | Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-01 01:16:30 | 0 | |
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pYTK083 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65190 | AmpR-ColE1 | Synthetic | Ampicillin | PMID:25871405 | Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:16:30 | 4 | |
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pYTK085 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65192 | SpecR-ColE1 | Synthetic | Spectinomycin | PMID:25871405 | Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2026-08-01 01:16:38 | 2 | |
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pYTK084 Resource Report Resource Website |
RRID:Addgene_65191 | KanR-ColE1 | Synthetic | Kanamycin | PMID:25871405 | Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-01 01:16:30 | 0 | |
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pcDNA3.1 mCherry Gamma 3 Resource Report Resource Website |
RRID:Addgene_65234 | mcherry | Ampicillin | PMID:24988358 | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:16:30 | 0 | |||
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shCTL-mlpx-puro Resource Report Resource Website |
RRID:Addgene_65232 | non-coding shRNA | Homo sapiens | Ampicillin | PMID:23599344 | Backbone Size:6533; Vector Backbone:pMLPX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:16:30 | 0 |
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