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Species: Saccharomyces cerevisiae
Genetic Insert: tSSA1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4 parts are terminators in the Dueber YTK For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65159 Copy
Species: Synthetic
Genetic Insert: GFP dropout
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 234r is used for the construction of dropout vectors. This plasmid encodes for a fluorescent dropout. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65154 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3b parts are used for coding sequences. This plasmid encodes for a fluorescent protein. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65153 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3001; Vector Backbone:pGEM5Z(+); Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19248754
Comments: To be used in conjunction with pGEM5Z(+)-EGFP (p111): www.addgene.org/65206. See corrigendum/erratum for original publication (https://doi.org/10.1016/j.ab.2009.10.024) before performing experiments with this plasmid.
Proper citation: RRID:Addgene_65207 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tADH1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4 parts are terminators in the Dueber YTK For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65160 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tENO2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4 parts are terminators in the Dueber YTK For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65162 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tENO1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4b parts are terminators used when a type 4a has been included as a coding sequence. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65168 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4a parts are used for C-terminal coding sequences. This plasmid encodes for a C-terminal fluorescent protein tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65164 Copy
Species: Synthetic
Genetic Insert: 6XHis_3XFlag
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4a parts are used for C-terminal coding sequences. This plasmid encodes for a C-terminal protein purification tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65167 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2 5' Homology
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Combination of part Types 7, 8a, and 8b are used for Yeast Chromosomal Integration in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65200 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tENO2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4b parts are terminators used when a type 4a has been included as a coding sequence. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65172 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tPGK1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4b parts are terminators used when a type 4a has been included as a coding sequence. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65171 Copy
Species: Synthetic
Genetic Insert: ConR1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65174 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tTDH1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4b parts are terminators used when a type 4a has been included as a coding sequence. For more information, please visit the supplemental information in PMID:25871405.
This plasmid stock has been found to contain dimers. Dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_65173 Copy
Species: Synthetic
Genetic Insert: ConRE
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65179 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6822.
VP1 capsid ORF utilizes a non-canonical initiation codon (TTG codon) beginning at nt 6514.
Additional article references: Barbash et al. Gene Therapy 20: 274-282 (2013).
Proper citation: RRID:Addgene_65215 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6824.
VP1 capsid ORF utilizes a non-canonical initiation codon (ACG codon) beginning at nt 6511.
Proper citation: RRID:Addgene_65214 Copy
Species: Synthetic
Genetic Insert: ConR3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65176 Copy
Species: Homo sapiens
Genetic Insert: esr1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_65211 Copy
Species: Homo sapiens
Genetic Insert: shRNA against ERK2
Vector Backbone Description: Backbone Size:6533; Vector Backbone:pMLPX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23599344
Proper citation: RRID:Addgene_65228 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
