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Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1
Vector Backbone Description: Backbone Size:6267; Vector Backbone:pRS416 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18685 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18601 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1 153-405
Vector Backbone Description: Backbone Size:6267; Vector Backbone:pRS416 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18689 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18602 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18603 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18605 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18608 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18613 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18616 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18617 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18619 Copy
Species: Homo sapiens
Genetic Insert: 8-oxoguanine DNA glycosylase
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pMCV/myc/nuc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17018150
Proper citation: RRID:Addgene_18709 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:MSCV Luciferase PGK Hygro; Vector Types:Mammalian Expression, Retroviral, Luciferase; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18782 Copy
Genetic Insert: H-ferritin iron response element
Vector Backbone Description: Backbone Marker:Available at Addgene (#18673); Backbone Size:6270; Vector Backbone:pYIC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17023487
Comments: An oligonucleotide with one copy of H-ferritin iron response element, IRE, was inserted into the 5'UTR of pYIC DNA to generate a bicistronic reporter gene with IRE: The IRE was inserted at the NheI and SacI sites (pIRE-YIC; 15 nt downstream of transcription start site and 9 nt upstream of YFP translational start site).
Proper citation: RRID:Addgene_18784 Copy
Species: Bacillus subtilis
Genetic Insert: TRAP
Vector Backbone Description: Backbone Size:4200; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17023487
Comments: Mammalian expression vector for the expression of HA and His6 epitope-tagged TRAP. There is a single amino acid (glycine) insertion between translational initiator codon and second amino acid of TRAP (asparagine) to maintain Kozak consensus sequence. Also, there is a single amino acid difference at amino acid #60 which has an asparagine (N) as in Bacillus amyloliquefaciens rather than glutamic acid (E) as in Bacillus subtilis. Codons are a mixture of human, Bacillus amyloliquefaciens, and Bacillus subtilis.
Proper citation: RRID:Addgene_18785 Copy
Genetic Insert: TRAP binding site
Vector Backbone Description: Backbone Marker:Available at Addgene (#18673); Backbone Size:6270; Vector Backbone:pYIC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17023487
Comments: An oligonucleotide with a 55-nt TRAP-binding sequence, TBS, was inserted into the 5'UTR of pYIC DNA to generate a bicistronic reporter gene with TBS: The TBS was inserted in the SacI site for 5-UTR (pTBS/5Y-YIC; 45 nt downstream of transcription start site and 9 nt upstream of YFP translational start site).
Proper citation: RRID:Addgene_18786 Copy
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6932; Vector Backbone:pFlag-CMV-2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pFLAG-CMV-2 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal FLAG tag.
Proper citation: RRID:Addgene_18700 Copy
Species: Mus musculus
Genetic Insert: epidermal growth factor receptor
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6280; Vector Backbone:pFLAG-CMV3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15020428
Comments: The EGFR internal signal sequence was removed during the cloning of this construct. When compared to GenBank reference sequence NP_997538.1, the signal sequence corresponds to amino acids 1-24
Proper citation: RRID:Addgene_18788 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7755; Vector Backbone:pcDNA3.1(-)/Myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pcDNA3.1(-)/myc-His B Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an C-terminal myc-His tag.
Proper citation: RRID:Addgene_18701 Copy
Species: Mus musculus
Genetic Insert: Type II transmembrane serine protease 6
Vector Backbone Description: Backbone Size:6265; Vector Backbone:p3xFLAG-CMV-14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18451267
Proper citation: RRID:Addgene_18789 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
