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Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike protein
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:2900; Vector Backbone:pRSET5A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35123472
Proper citation: RRID:Addgene_171709 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18574 Copy
Species: Homo sapiens
Genetic Insert: Raptor
Vector Backbone Description: Backbone Marker:BD Pharmingen; Backbone Size:4754; Vector Backbone:pRK-5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15268862
Proper citation: RRID:Addgene_1859 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:8331; Vector Backbone:pUC18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15655804
Proper citation: RRID:Addgene_18660 Copy
Species: Synthetic DNA
Genetic Insert: TREPitt
Vector Backbone Description: Backbone Size:2812; Vector Backbone:pLitmus 29; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15655804
Proper citation: RRID:Addgene_18661 Copy
Genetic Insert: Ras binding domain of Raf
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16429133
Proper citation: RRID:Addgene_18664 Copy
Species: Homo sapiens
Genetic Insert: H-Ras
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16429133
Proper citation: RRID:Addgene_18665 Copy
Species: Homo sapiens
Genetic Insert: thioredoxin-like 4B
Vector Backbone Description: Backbone Size:2930; Vector Backbone:pBZ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18064608
Comments: attB cassette flanking coding region of TXNL4B (dim2) can be released by digestion with NotI and BamHI
Proper citation: RRID:Addgene_18667 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:7934; Vector Backbone:pET19b; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pET19b Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal His tag. Note that the Addgene verified sequence detects a mutation in the enterokinase site D(V)DDK.
Proper citation: RRID:Addgene_18668 Copy
Genetic Insert: ERK Activity Reporter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19033456
Proper citation: RRID:Addgene_18680 Copy
Vector Backbone Description: Backbone Size:7040; Vector Backbone:pEGFPC4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:18064608
Comments: pEGFPC4 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal EGFP tag.
Proper citation: RRID:Addgene_18671 Copy
Species: Homo sapiens
Genetic Insert: Bicistronic fluorescent reporter gene with cap-dependent EYFP and IRES-dependent ECFP translation under the control of CMV promo
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17023487
Comments: EYFP tagged at N-terminus with 3 copies of Myc epitope tags and at C-terminus with a copy of FLAG and polyhistidine (His6) epitope tags. ECFP tagged at the C-terminus with one copy of hemagglutinin (HA) and polyhistidine (His6) epitope tags.
EGFP of pIRES2-EGFP (Clontech) replaced at an 803 bp BstXI-NotI, PCR amplified fragment with ECFP from pECFP-Mito (Clontech) tagged at the C-terminus with a single copy of hemagglutinin (HA) and polyhistidine (His6) epitope tags; IRES-dependent translation yields ECFP-HA-His6 fusion protein. 895 bp Sac I/Eco RI fragment containing amino terminal 3 Myc epitope tags fused to EYFP obtained from PCR amplification of pEYFP-N1 (Clontech), which is also tagged at C-terminus with a single copy of FLAG and polyhistidine (His6) epitope tag; cap-dependent translation yields 3Myc-EYFP-Flag-His6 fusion protein.
See article for detailed information.
Proper citation: RRID:Addgene_18673 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18640 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18641 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18645 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18646 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18648 Copy
Species: Homo sapiens
Genetic Insert: Cdk6
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV-neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8266103
Comments: See scanned map.
Proper citation: RRID:Addgene_1868 Copy
Species: Homo sapiens
Genetic Insert: Cdk6
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV-neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8266103
Comments: See scanned map.
Proper citation: RRID:Addgene_1869 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18650 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
