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Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI (ORF)
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3 parts are generally reserved for coding sequences in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65142 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal fluorescent protein tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65144 Copy
Genetic Insert: ecfp
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:17406227
Comments: GenBank Accession # DQ493878
Proper citation: RRID:Addgene_65030 Copy
Genetic Insert: gfpmut3
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Zeo; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Bleocin (Zeocin)
Defining Citation: PMID:17406227
Comments: GenBank Accession # DQ493885
Please note that Addgene's sequencing results (and the updated full plasmid sequence listed above) differ from the GenBank sequence, including a R80Q mutation in gfpmut3. The effect of these differences is unknown.
Proper citation: RRID:Addgene_65037 Copy
Genetic Insert: eyfp
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Tp; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17406227
Comments: GenBank Accession # DQ493883
Proper citation: RRID:Addgene_65035 Copy
Genetic Insert: dsRedExpress
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Zeo; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Bleocin (Zeocin)
Defining Citation: PMID:17406227
Comments: GenBank Accession # DQ493888
Please note that Addgene's sequencing results (and the updated full plasmid sequence listed above) differ from the GenBank sequence. The effect of these differences is unknown.
Proper citation: RRID:Addgene_65040 Copy
Species: Homo sapiens
Genetic Insert: Engineered target sites miR-148a-3p
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4637; Vector Backbone:pGL4.13; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24895996
Proper citation: RRID:Addgene_65053 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-148a (mutated)
Vector Backbone Description: Backbone Size:5175; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24895996
Comments: Please note that this plasmid was also described in PubMed ID 25714032.
Proper citation: RRID:Addgene_65055 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-216a (pre-miRNA)
Vector Backbone Description: Backbone Size:5175; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24895996
Proper citation: RRID:Addgene_65054 Copy
Species: Rattus norvegicus
Genetic Insert: DDIT4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pCMS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17005863
Comments: There are 2 mutations if compared to PubMed DDIT4 R. Norvegicus sequence (L121V and S208G) because the insert was cloned out of DC12 cells DNA.
Proper citation: RRID:Addgene_65057 Copy
Species: Mus musculus
Genetic Insert: PRMT7
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Bacterial Propagation; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24247247
Comments: Addgene sequencing results found an F4S amino acid change in the PRMT7 translation relative to genbank ID NM_145404.1.
Proper citation: RRID:Addgene_65056 Copy
Species: Homo sapiens
Genetic Insert: Delta(Pro-MP)ADAM10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12743035
Comments: Mutagenesis creates EcoRI site 57bp downstream of Start ATG
Proper citation: RRID:Addgene_65107 Copy
Species: Rattus norvegicus
Genetic Insert: shNP1
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11090; Vector Backbone:pLVTH; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25855168
Proper citation: RRID:Addgene_65062 Copy
Species: Rattus norvegicus
Genetic Insert: Neuronal Pentraxin 1
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:10510; Vector Backbone:pWPXL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25855168
Comments: The mutations introduce a new EcoR1 restriction site on bp 459 of CDS to allow distinction between wildtype and resistant NP1. However, this prevents excision of the whole resNP1 insert from the pWPXL vector using EcoR1 restriction site
Proper citation: RRID:Addgene_65061 Copy
Species: Rattus norvegicus
Genetic Insert: Neuronal Pentraxin 1
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11078; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25855168
Comments: * N280D and R322Q are present in NP1 and this plasmid should function as described in the associated publication.
Proper citation: RRID:Addgene_65060 Copy
Species: Synthetic
Genetic Insert: MS2
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pMal-c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18570876
Comments: The following reference describes how to isolate RNA binding complexes using this construct:
Jurica and Moore, Methods. 2002 Nov;28(3):336-45. https://www.ncbi.nlm.nih.gov/pubmed/12431437
Proper citation: RRID:Addgene_65104 Copy
Genetic Insert: ABKAR biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25892241
Proper citation: RRID:Addgene_65068 Copy
Genetic Insert: RBD, mCerulean, mVenus, RhoC
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24224016
Proper citation: RRID:Addgene_65071 Copy
Species: Synthetic
Genetic Insert: ConL5
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 1 parts are 5' assembly connectors in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65114 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTDH3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 2 parts are promoters in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65116 Copy
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