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Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 S protein
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33243994
Comments: Pseudotypes to the MLV vector but not efficiently to VSV or lentiviral vectors.
Please visit https://www.biorxiv.org/content/10.1101/2020.06.12.148726v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_159363 Copy
Species: Synthetic
Genetic Insert: TAX-L10-STEPtag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5677; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33044070
Comments: TAX can be replaced with a protein of interest using the NdeI and XhoI sites. Ensure that the flexible ten amino acid linker between STEPtag and the protein of interest is present in the new fusion.
Proper citation: RRID:Addgene_159375 Copy
Species: Synthetic
Genetic Insert: gSTEP1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5301; Vector Backbone:pET-Duet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33044070
Proper citation: RRID:Addgene_159376 Copy
Species: Synthetic
Genetic Insert: STEPtag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5677; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33044070
Proper citation: RRID:Addgene_159373 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159306 Copy
Species: Synthetic
Genetic Insert: tdKOkappa
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159307 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159305 Copy
Species: Synthetic
Genetic Insert: mAmetrine
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159302 Copy
Species: Synthetic
Genetic Insert: TALEN targeting the AAVS1
Vector Backbone Description: Backbone Size:4514; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159300 Copy
Species: Synthetic
Genetic Insert: mTFP1
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Insert is flanked/contains AgeI and NgoMIV sites to insert copy multiplication.
Proper citation: RRID:Addgene_159301 Copy
Species: Homo sapiens
Genetic Insert: Talin Rod Domain Containing Protein 1
Vector Backbone Description: Backbone Size:5760; Vector Backbone:pET151; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34264272
Proper citation: RRID:Addgene_159384 Copy
Species: Homo sapiens
Genetic Insert: KRAS G12D
Vector Backbone Description: Vector Backbone:pcDNA2.0; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30952657
Proper citation: RRID:Addgene_159439 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32411820
Proper citation: RRID:Addgene_159436 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32411820
Proper citation: RRID:Addgene_159437 Copy
Species: Other
Genetic Insert: miRFP670
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32411820
Proper citation: RRID:Addgene_159435 Copy
Species: Synthetic
Genetic Insert: pr.tac-sfGFP
Vector Backbone Description: Backbone Marker:iGEM parts registry; Vector Backbone:pSB1A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32948587
Proper citation: RRID:Addgene_159397 Copy
Species: Mus musculus
Genetic Insert: gRNA library targeting mouse genes
Vector Backbone Description: Backbone Marker:Jason Moffat; Vector Backbone:pLCKO2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32968282
Proper citation: RRID:Addgene_159393 Copy
Species: Mus musculus
Genetic Insert: pr.tac-BDNF (Bdnf, Mouse)
Vector Backbone Description: Backbone Marker:iGEM parts registry; Vector Backbone:pSB1A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32948587
Proper citation: RRID:Addgene_159394 Copy
Species: Synthetic
Genetic Insert: PlexAtet-Citrine-tCyc1
Vector Backbone Description: Backbone Marker:addgene; Backbone Size:7471; Vector Backbone:pRG207; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33767179
Proper citation: RRID:Addgene_159447 Copy
Species: Synthetic
Genetic Insert: P2A-iCre
Vector Backbone Description: Backbone Size:6600; Vector Backbone:PL652; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32966778
Proper citation: RRID:Addgene_159459 Copy
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