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Species: Homo sapiens
Genetic Insert: Nb127D01-GFP
Vector Backbone Description: Vector Backbone:pAW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35076390
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440240v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_171571 Copy
Species: Homo sapiens
Genetic Insert: NbVHH05-GFP
Vector Backbone Description: Vector Backbone:pAW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35076390
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440240v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_171570 Copy
Species: Homo sapiens
Genetic Insert: H2B
Vector Backbone Description: Vector Backbone:pGEHME; Vector Types:pGEHME; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33964210
Proper citation: RRID:Addgene_171489 Copy
Species: Mus musculus
Genetic Insert: MAP4-MTBD
Vector Backbone Description: Vector Backbone:pGEHME; Vector Types:pGEHME; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33964210
Comments: This is the Microtubule binding domain of MAP4.
5' cloning site: XhoI (unknown if destroyed), 3' cloning site: XbaI (unknown if destroyed).
Proper citation: RRID:Addgene_171488 Copy
Species: Other
Genetic Insert: GST
Vector Backbone Description: Vector Backbone:pGEHME; Vector Types:pGEHME; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33964210
Proper citation: RRID:Addgene_171486 Copy
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:0; Vector Backbone:pCS2+mRFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Notes for Use:
For making amino-terminal mRFP fusion protein, reading frame 1, in vector CS2+.
Made by: Jeff Miller.
Proper citation: RRID:Addgene_17143 Copy
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:0; Vector Backbone:pCS2+mRFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Notes for Use:
For making amino-terminal mRFP fusion protein, reading frame 3, in vector CS2+.
Made by: Jeff Miller.
Proper citation: RRID:Addgene_17145 Copy
Species: Xenopus laevis
Genetic Insert: Beta-tubulin promoter
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4300; Vector Backbone:pBLCAT3; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Constructed Rebecca Beach and Paul A. Kreig.
3.8Kb fragment of the Xenopus neural Beta-tubulin promoter inserted (in several cloning steps) into the CAT expression vector pBLCAT3. The 5' end of the neural Beta-tubulin sequence is defined by the natural Xba1 site that occurs in the genomic DNA. The 3' end of the insert is produced by PCR primer located 8bp upstream of hte initiation ATP and 120bp downstream of the transcription start site. The neural Beta-tubulin sequences may be excised as a Hind3 fragment.
Proper citation: RRID:Addgene_17146 Copy
Species: Xenopus laevis
Genetic Insert: NCAM promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Comments: Plasmid History:
Constructed Paul A. Kreig, July.
Note for Use:
This construction contains a 1.3 Kb Dra I fragment of the NCAM promoter and should be okay for general neural expression. The fragment is cloned into the Sma I site of Bluescript KS+. The NCAM promoter does not contain a TATA box and transcription starts at numerous sites over about 350 bases. All of the potential start sites are contained in this 1.3 Kb fragment, which ends in the 5' UTR of the NCAM gene. The Dra I fragment starts at the very beginning of the GenBank sequence (starting with aaa) and ends at base 1358 of the GenBank sequence.
Proper citation: RRID:Addgene_17147 Copy
Species: Xenopus laevis
Genetic Insert: cardiac actin promoter
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCSKA; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Comments: Plasmid History:
Constructed by Enrique Amaya.
Note for Use:
Constructed by cloning the 3270bp KpnI-SalI fragment from DNA#254 (from Tim Mohun) into KpnI-SalI digested pCSKA.
Proper citation: RRID:Addgene_17148 Copy
Species: Xenopus laevis
Genetic Insert: cardiac actin promoter
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCSKA; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Comments: Plasmid History:
Constructed by Enrique Amaya.
Note for Use:
Generated by inserting the 1Kb HindIII-NotI fragment from pCSGFP2 into HindIII-NotI digested pCarG.
This GFP constuct has been modified by Jim Haseloff and Jonathan Pines to be brighter and more soluble that wild-type GFP.
Note: Uniques sites are underlined in Author's Map.
Proper citation: RRID:Addgene_17149 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnt1
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751023
Proper citation: RRID:Addgene_171549 Copy
Species: Synthetic
Genetic Insert: HaloTag N58-(GGS)X6-FRB
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33742089
Proper citation: RRID:Addgene_171427 Copy
Species: Xenopus laevis
Genetic Insert: Xlrbpa
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751023
Proper citation: RRID:Addgene_171548 Copy
Species: Homo sapiens
Genetic Insert: ATG14
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33893090
Proper citation: RRID:Addgene_171426 Copy
Species: Xenopus laevis
Genetic Insert: Xlrbpa
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751023
Proper citation: RRID:Addgene_171547 Copy
Species: Homo sapiens
Genetic Insert: OPTN
Vector Backbone Description: Vector Backbone:pGST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33893090
Proper citation: RRID:Addgene_171424 Copy
Species: Homo sapiens
Genetic Insert: NDP52
Vector Backbone Description: Vector Backbone:pGST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33893090
Proper citation: RRID:Addgene_171422 Copy
Species: Other
Genetic Insert: Ubiquitin
Vector Backbone Description: Vector Backbone:pGEX5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33893090
Proper citation: RRID:Addgene_171421 Copy
Vector Backbone Description: Backbone Size:6420; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 2005. Fire Lab Miniprep Number pPD158.114, Ligation number L5784.
Proper citation: RRID:Addgene_1715 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
