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Species: Homo sapiens
Genetic Insert: NbVHH05-HA-His
Vector Backbone Description: Vector Backbone:pET-26b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35076390
Comments: Cloned by HiFi assembly.
Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440240v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_171567 Copy
Species: Homo sapiens
Genetic Insert: Nb127D01-HA-His
Vector Backbone Description: Vector Backbone:pET-26b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35076390
Comments: Cloned by Hifi assembly.
Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440240v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_171566 Copy
Species: Homo sapiens
Genetic Insert: NbVHH05-human IgG-TEV-Avi-His
Vector Backbone Description: Backbone Marker:Ah-Ram Kim (Perrimon Lab); Vector Backbone:pMT-HGP-v3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35076390
Comments: This is extensively engineered vector. Originally, HGP (heat shock promoter-GFP-T2A-promoter) was added into pAW vector by Gibson cloning, resulting in pAW-HGP vector. And then, the cloning part including N-term Signal peptide / FLAG peptide and C-term TEV site/Avi-tag/His-tag) was introduced into pAW-HGP vector, resulting in pAW-HGP-v2. Finally, the Actin promoter was replaced with pMT promoter, resulting in final vector pMT-HGP-v3.
Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440240v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_171565 Copy
Species: Synthetic
Genetic Insert: HaloTag N58-(GGS)X6-c-Jun
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33742089
Proper citation: RRID:Addgene_171438 Copy
Species: Synthetic
Genetic Insert: a sequences perfectly complementary to miR-124
Vector Backbone Description: Vector Backbone:pCMV-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31366019
Proper citation: RRID:Addgene_171558 Copy
Species: Synthetic
Genetic Insert: Lifeact-(GGS)X6-HaloTag C58
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33742089
Proper citation: RRID:Addgene_171435 Copy
Species: Synthetic
Genetic Insert: a sequences perfectly complementary to miR-1
Vector Backbone Description: Vector Backbone:pCMV-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31366019
Proper citation: RRID:Addgene_171556 Copy
Species: Synthetic
Genetic Insert: tdMCP2-GyG-(GGS)X6-HaloTag C261
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33742089
Proper citation: RRID:Addgene_171434 Copy
Species: Homo sapiens
Genetic Insert: miR-124
Vector Backbone Description: Vector Backbone:pSuper; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31366019
Proper citation: RRID:Addgene_171555 Copy
Species: Synthetic
Genetic Insert: hH2A-(GGS)X6-HaloTag C58
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33742089
Proper citation: RRID:Addgene_171431 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnt1
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751023
Proper citation: RRID:Addgene_171552 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnt1
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751023
Proper citation: RRID:Addgene_171550 Copy
Species: Homo sapiens
Genetic Insert: ActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-mCherry
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmCherry-C1 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35475143
Proper citation: RRID:Addgene_171465 Copy
Species: Danio rerio
Genetic Insert: twhh promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCS-NBeta gal; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314535
Comments: Insert: twhh promoter (5.2 Kb), nuclear Beta gal reporter (3 Kb)
Plasmid History:
Source: zebrafish.
Notes for Use:
Gives notochord-specific expression of Beta-gal.
Citation: Du et al., 1997, J. Cell Biology.
Proper citation: RRID:Addgene_17152 Copy
Species: Danio rerio
Genetic Insert: twhh promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:NA; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Insert: twhh promoter (5.2 Kb), rabbit Beta-globin intron 2 (IVS2, 640 bp), GFP (65C, 1Kb)
Plasmid History:
Source: zebrafish.
Notes for Use:
Gives both notochord and floor-plate specific expression of GFP, owing to having the intron present.
Proper citation: RRID:Addgene_17154 Copy
Species: Xenopus laevis
Genetic Insert: siamois promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9308964
Comments: Plasmid History:
Constructed by David Kimelman, 6/6/96 XVII p. 98.
Notes for Use:
The -3.0kb-siamois promoter was directionally cloned into the KpnI and BglII sites of the luciferase reporter vector pGL3B (Promega) upstream of the luciferase ATG. The -0.8kb promoter construct was generated by digesting the -3.0kb/pGL3B construct with KpnI and at an internal EcoRI site to remove 2.2 kb of intervening sequence, filling in with DNA polymerase I large fragment and religating.
The Kpn site was lost but the R1 site was regenerated in the ligation. The size of the insert is approximate.
Citation: Brannon et al., Gene and Development 11:2359-2370, 1997.
Proper citation: RRID:Addgene_17157 Copy
Species: Homo sapiens
Genetic Insert: mCherry-FKBP12-Caveolin1(61-178 aa)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmCherry-C1 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35475143
Comments: Please note: The plasmid contains a D220E mutation in mCherry compared to the depositor's sequence. This mutation does not affect plasmid function.
Proper citation: RRID:Addgene_171464 Copy
Species: Homo sapiens
Genetic Insert: ActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-EYFP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEYFP-C1 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35475143
Proper citation: RRID:Addgene_171463 Copy
Species: Homo sapiens
Genetic Insert: Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pECFP-C1 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35475143
Proper citation: RRID:Addgene_171461 Copy
Species: Homo sapiens
Genetic Insert: EYFP(A207K)-FKBP12
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEYFP-C1 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35475143
Proper citation: RRID:Addgene_171460 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
