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Species: Xenopus laevis
Genetic Insert: full coding of Xwnt-5A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Insert: full coding of Xwnt-5A.
Insert size: 1.2 Kb.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by RTM/JR on the date of 4/93.
Notes for Use:
This construct encodes full coding sequence of Xwnt-5A, without wnt UTR's.
See Xwnt 5A full-length for Genbank data.
Proper citation: RRID:Addgene_16874 Copy
Species: Xenopus laevis
Genetic Insert: Wnt-5A
Vector Backbone Description: Backbone Marker:Available from Addgene (#17120); Backbone Size:0; Vector Backbone:pCD-betaG-PS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The myc tagged Xwnt-5A insert of clone XE40, in the SV40 construct pCD-PS modified by Julia Yang-Snyder to include Xenopus beta globin flanking untranslated regions.
Reference: The clone was made by Julia Yang-Snyder.
Addgene QC found a G125A point mutation that is not known to affect function.
Proper citation: RRID:Addgene_16873 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin Conjugating Enzyme 9
Vector Backbone Description: Backbone Size:13218; Vector Backbone:pInducer-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25805818
Proper citation: RRID:Addgene_168987 Copy
Species: Xenopus laevis
Genetic Insert: dnWnt-11
Vector Backbone Description: Backbone Marker:Available at Addgene (#15030); Backbone Size:3300; Vector Backbone:pSP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10769246
Comments: A C-terminally truncated dominant-negative Xwnt11 construct (comprising amino acids 1-282) was generated according to Hoppler
et al. (1996). PCR was carried out using pGEM-X9 (Ku and Melton, 1993) as a template and the primers 5′-CCCCCTCGAGAGTACCAATGGCTCCGACCCG-3′ and 5′-TTGGAGATCTTTCAGCAGTAGTCAGGGGAAC-3′. The PCR product obtained was cloned into the XhoI and BglII sites of pSP64TXB.
For injection, linearize with BamH1 and transcribe with SP6.
Proper citation: RRID:Addgene_16857 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin Conjugating Enzyme 9
Vector Backbone Description: Backbone Size:13217; Vector Backbone:pInducer-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25805818
Proper citation: RRID:Addgene_168988 Copy
Species: Homo sapiens
Genetic Insert: SRGAP1-FBAR
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22467852
Proper citation: RRID:Addgene_168505 Copy
Species: Mus musculus
Genetic Insert: Wnt-1-myc
Vector Backbone Description: Backbone Size:2746; Vector Backbone:pGEM4; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2673541
Comments: Description: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope. Protein can be immunoprecipitated, recognized in whole mount immunostaining, and detected by western blot, using the anti-c-myc antibody 9E-10.
Species of Sequence Origin: mouse.
Notes for Use: Linearize with Eco R1 and transcribe with T7 to make RNA.
Reference: This is the clone published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989).
Proper citation: RRID:Addgene_16855 Copy
Species: Homo sapiens
Genetic Insert: SUMO1 Activating Enzyme Subunit 1
Vector Backbone Description: Backbone Size:13217; Vector Backbone:pInducer-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25805818
Proper citation: RRID:Addgene_168984 Copy
Species: Homo sapiens
Genetic Insert: SUMO1 Activating Enzyme Subunit 1
Vector Backbone Description: Backbone Size:13217; Vector Backbone:pInducer-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25805818
Proper citation: RRID:Addgene_168985 Copy
Species: Mus musculus
Genetic Insert: Wnt-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, cloned into the vector pCS2+
Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16851 Copy
Vector Backbone Description: Backbone Size:17590; Vector Backbone:pYFAC-pyroA; Vector Types:Synthetic Biology, Heterologous expression in Aspergillus nidulans and related filamentous fungi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30809363
Comments: A detailed protocol on how to use this plasmid for fungal biosynthetic gene cluster elucidation projects can be found in Roux I., Chooi Y.H. (2022) Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_5
Proper citation: RRID:Addgene_168980 Copy
Species: Homo sapiens
Genetic Insert: SRGAP3-FBAR
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22467852
Proper citation: RRID:Addgene_168506 Copy
Species: Mus musculus
Genetic Insert: Nuak1
Vector Backbone Description: Vector Backbone:pCIG2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23791179
Proper citation: RRID:Addgene_168507 Copy
Vector Backbone Description: Vector Backbone:pETconNK; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34416153
Proper citation: RRID:Addgene_168778 Copy
Species: betacoronavirus
Genetic Insert: Spike RBD
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34416153
Proper citation: RRID:Addgene_168779 Copy
Species: Synthetic
Genetic Insert: mEos3.2_H-NSdbd
Vector Backbone Description: Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_168774 Copy
Species: Synthetic
Genetic Insert: mEos3.2
Vector Backbone Description: Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_168775 Copy
Species: Danio rerio
Genetic Insert: Hrasls
Vector Backbone Description: Vector Backbone:Tol2-aACry; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854238
Proper citation: RRID:Addgene_168772 Copy
Species: Synthetic
Genetic Insert: Fluorogen activating protein DiB3
Vector Backbone Description: Backbone Size:3958; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29147545
Proper citation: RRID:Addgene_168802 Copy
Species: Homo sapiens
Genetic Insert: Rab5-binding domain (aa739-862) of Rabaptin-5
Vector Backbone Description: Backbone Marker:Pharmacia/GE Healthcare; Backbone Size:4970; Vector Backbone:pGEX-4T-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25800849
Proper citation: RRID:Addgene_168808 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
