Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Homo sapiens
Genetic Insert: AR-AAFF
Vector Backbone Description: Backbone Size:10000; Vector Backbone:Lenti-viral-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34911936
Comments: The mutations occur on the androgen receptor KVFF motif in the P-box of the zinc finger 1 that make contact with DNA. AR mutants of the KVFF motif would lose activity to activate AR-mediated transcription.
Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
Proper citation: RRID:Addgene_171218 Copy
Species: Homo sapiens
Genetic Insert: M-SPOTIT1.1
Vector Backbone Description: Backbone Size:6783; Vector Backbone:PLX208; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33662184
Proper citation: RRID:Addgene_171216 Copy
Species: Homo sapiens
Genetic Insert: K-SPOTIT1.0
Vector Backbone Description: Backbone Size:6778; Vector Backbone:PLX208; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33662184
Proper citation: RRID:Addgene_171215 Copy
Species: Synthetic
Genetic Insert: hAAVS1 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:5148; Vector Backbone:pBBR1-MCS2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK041.AAV, pBBR1-KmR_Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A/S101A)_AAV
Proper citation: RRID:Addgene_171175 Copy
Species: Other
Genetic Insert: dTomato
Vector Backbone Description: Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_171174 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22275373
Comments: Flag-tagged human AR Ser-Pro phosphorylation mutant that binds to bicalutamide, and the exact amino position and composition may slightly vary based on human AR versions.Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171249 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Trp741Cys mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22275373
Comments: Flag-tagged human AR mutant that binds to bicalutamide, and the exact amino position and composition may slightly vary based on human AR versions.Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171247 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Ser650Asp mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: Mutation in Flag-tagged AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171246 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Ser650Ala mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: Mutation in Flag-tagged AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171245 Copy
Species: Homo sapiens
Genetic Insert: 1xHA-tagged human androgen receptor trancate:Ligand-binding domain
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1(+)-kozakHAHA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: It is based on a single HA tagged vector.
Proper citation: RRID:Addgene_171239 Copy
Species: Homo sapiens
Genetic Insert: 2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1(+)-kozakHAHA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: For cloning purpose, an "A" nucleotide was inserted right after BamHI site in the vector backbone by DNA mutagensis to change the open reading frame.
Proper citation: RRID:Addgene_171237 Copy
Species: Homo sapiens
Genetic Insert: 2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1(+)-kozakHAHA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: For cloning purpose, an "A" nucleotide was inserted right after BamHI site in the vector backbone by DNA mutagensis to change the open reading frame.
Proper citation: RRID:Addgene_171236 Copy
Species: Synthetic
Genetic Insert: J306 scRNA
Vector Backbone Description: Backbone Marker:Jesse Zalatan; Backbone Size:6261; Vector Backbone:pPPC020.306; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK134, pBBR1-GmR_J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
Proper citation: RRID:Addgene_171149 Copy
Species: Homo sapiens
Genetic Insert: 3x-Flag-tagged human PP1 beta catalytic subunit
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The construction is based on PCR amplification using Pfu DNA polymerase.
Proper citation: RRID:Addgene_171269 Copy
Species: Synthetic
Genetic Insert: hAAVS1 scRNA
Vector Backbone Description: Backbone Marker:Jesse Zalatan; Backbone Size:4577; Vector Backbone:pRK2-KmR; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK080, pRK2-KmR_J1-BBa_J23117-mRFP_hAAVS1.
Proper citation: RRID:Addgene_171147 Copy
Species: Homo sapiens
Genetic Insert: 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: The construction is based on PCR amplification using Pfu DNA polymerase and DNA mutagenesis.
Proper citation: RRID:Addgene_171268 Copy
Species: Homo sapiens
Genetic Insert: 3x-Flag-tagged human PP1 alpha catalytic subunit
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: The construction is based on PCR amplification using Pfu DNA polymerase.
Proper citation: RRID:Addgene_171267 Copy
Species: Synthetic
Genetic Insert: J109 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:5148; Vector Backbone:pBBR1-MCS2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK058, pBBR1-KmR_J1-mRFP_J109
Proper citation: RRID:Addgene_171145 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Lys639Ala mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Flag-tagged human AR Lysine mutant, and the exact amino position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171264 Copy
Species: Synthetic
Genetic Insert: J106 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:5148; Vector Backbone:pBBR1-MCS2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK033.06, pBBR1-KmR_J106
Proper citation: RRID:Addgene_171142 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the kravitz2 Resources search. From here you can search through a compilation of resources used by kravitz2 and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that kravitz2 has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on kravitz2 then you can log in from here to get additional features in kravitz2 such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into kravitz2 you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within kravitz2 that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.