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Species: Homo sapiens
Genetic Insert: RhoA
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10087266
Comments: There is an additional F25N mutation in the sequence. This change greatly facilitated expression of the protein in E.coli. The only known effect of this mutation may be negatively affect the transduction of RhoA signaling to DGK1 kinase. Otherwise the depositing lab have never found any discrepancies with other published work on activity or protein interactions.
Proper citation: RRID:Addgene_15899 Copy
Species: Homo sapiens
Genetic Insert: GSK3 beta
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: ORF is at 258-2237.
The Addgene Quality Control sequencing results have found silent nucleotide changes in the depositor GSK3 beta sequence as well as an amino acid change. The verified sequence matches PBD Sequence ID: 4IQ6_A and encodes an isoform of human GSK3 beta that should function as WT, please review the Addgene QC sequence before use.
Proper citation: RRID:Addgene_15898 Copy
Species: Homo sapiens
Genetic Insert: GSK3 alpha
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2164470
Comments: ORF is at 1189-2640.
Proper citation: RRID:Addgene_15896 Copy
Species: Synthetic
Genetic Insert: WP_104244666_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with WP_104244666_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18119
Proper citation: RRID:Addgene_158929 Copy
Species: Synthetic
Genetic Insert: WP_115933252_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with WP_115933252_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18115
Proper citation: RRID:Addgene_158927 Copy
Species: Synthetic
Genetic Insert: WP_070193933_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with WP_070193933_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18107
Proper citation: RRID:Addgene_158923 Copy
Vector Backbone Description: Backbone Size:3777; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD118.15, Ligation number L3781.
Proper citation: RRID:Addgene_1590 Copy
Species: Synthetic
Genetic Insert: WP_096909276_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with WP_096909276_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18135
Proper citation: RRID:Addgene_158937 Copy
Species: Synthetic
Genetic Insert: WP_108826550_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with WP_108826550_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18129
Proper citation: RRID:Addgene_158934 Copy
Species: Synthetic
Genetic Insert: WP_119951226_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with WP_119951226_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18131
Proper citation: RRID:Addgene_158935 Copy
Species: Homo sapiens
Genetic Insert: EMI1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:PCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30554948
Proper citation: RRID:Addgene_158947 Copy
Species: Synthetic
Genetic Insert: CVN04163_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with CVN04163_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18151
Proper citation: RRID:Addgene_158945 Copy
Species: Synthetic
Genetic Insert: OGN97459_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with OGN97459_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18147
Proper citation: RRID:Addgene_158943 Copy
Species: Synthetic
Genetic Insert: WP_005508112_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with WP_005508112_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18149
Proper citation: RRID:Addgene_158944 Copy
Species: Synthetic
Genetic Insert: WP_061782733_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with WP_061782733_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18143
Proper citation: RRID:Addgene_158941 Copy
Species: Synthetic
Genetic Insert: ABL00697_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with ABL00697_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18141
Proper citation: RRID:Addgene_158940 Copy
Species: Synthetic
Genetic Insert: BLRR
Vector Backbone Description: Backbone Size:7983; Vector Backbone:pLenti CMV Puro DEST (w118-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32797168
Proper citation: RRID:Addgene_158958 Copy
Species: Homo sapiens
Genetic Insert: trGluc
Vector Backbone Description: Backbone Size:8644; Vector Backbone:CSCW-IRES-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32797168
Proper citation: RRID:Addgene_158959 Copy
Species: Homo sapiens
Genetic Insert: RAD51
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcdna3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30554948
Proper citation: RRID:Addgene_158953 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0229
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159005 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
