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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pJexpress414_OmpA171_H2467
 
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RRID:Addgene_64294 OmpA_H2467 Synthetic Ampicillin PMID:26199411 Amino acid sequence: mkktaiaiavalagfatvaqaapndntFyLgLkMgIsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgLeLgVdVlgrmpykgsvengaykaqgVqLtAkLgYpitddVdVyLrLgImVwradtksnvygknhdtgvsPvFaGgVeYaItpeIaTrLeYqWtnnigdYPYDVPDYAtrpdnWmVsLgAsYrfgCCPGCCHHHHHH Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:16:22 0
pJexpress414_OmpA171_H1248
 
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RRID:Addgene_64293 OmpA_H1248 Synthetic Ampicillin PMID:26199411 Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgLeLgVdVlgrmpykgsvengaykaqgVqLtAkLgYpitddVdVyLrLgImVwradtksnvygknhdtgvsFvMaLgSeHaItpeIaMrLeTqHtnnigdYPYDVPDYAtrpdnGmLsLgVsYrfgCCPGCCHHHHHH Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:16:22 0
pBa-KIF5C 559-tdTomato-FKBP
 
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RRID:Addgene_64211 Kif5c Rattus norvegicus Ampicillin PMID:25624392 pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination. XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains. Backbone Marker:Banker lab (Addgene plasmid #45059); Backbone Size:8410; Vector Backbone:pBa.KIF5C 1-559.eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin motor domain aa1-559 2026-08-01 01:16:34 5
gRNA-leu-HYB
 
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RRID:Addgene_64332 gBlock product of leu2 deletion gRNA cassette Synthetic Ampicillin PMID:25281382 Backbone Size:6217; Vector Backbone:pRS42H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:16:22 1
Cry2 mCherry PGK-1 in pcDNA3.1
 
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RRID:Addgene_64214 Cry2 PHR Arabidopsis thaliana Ampicillin PMID:24920824 PGK1 is cloned within the KpnI and XbaI sites Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:16:34 0
6691 Bicistronic_ires_puro
 
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RRID:Addgene_64335 Ampicillin PMID:28508873 This construct was created in G. Stathopoulos lab in Patras, Greece, by Theodora Agalioti; The backbone is derived from MigR1 vector to which a partial IRES followed by puromycin selection gene derived from pPY_CAG_CRE_IP plasmid was inserted Backbone Marker:Theodora Agalioti; Vector Backbone:Migr1; Vector Types:Retroviral; Bacterial Resistance:Ampicillin 2026-08-01 01:16:35 0
pBa-FRB-3myc-Rab7
 
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RRID:Addgene_64210 Rab7 Mus musculus Ampicillin PMID:25624392 pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination. XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains. Backbone Size:6280; Vector Backbone:pBa-FRB-3myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:16:21 0
pJexpress414_OmpA171_R4cons_G125L
 
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RRID:Addgene_64298 OmpA_R4cons_G125L Synthetic Ampicillin PMID:26199411 Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:16:35 0
gRNA-trp-HYB
 
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1+ mentions
RRID:Addgene_64331 gBlock product of trp1 deletion gRNA cassette Synthetic Ampicillin PMID:25281382 Note: Addgene's quality control sequencing finds one mismatch in the SNR52 promoter region, but plasmid function is not impacted. Backbone Size:6217; Vector Backbone:pRS42H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:16:35 2
pJexpress414_OmpA171_H1358
 
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RRID:Addgene_64297 OmpA_H1358 Synthetic Ampicillin PMID:26199411 Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqQgIgAfAgIqVnpyVgFeMgYdWlgrmpykgsvengaykaqgYqAtLkMgVpitddLdIyTrLgGmVwradtksnvygknhdtgvsFvMaLgSeHaItpeIaMrLeTqHtnnigdYPYDVPDYAtrpdnGmLsLgVsYrfgCCPGCCHHHHHH Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:16:22 0
gRNA-ura-HYB
 
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RRID:Addgene_64330 gBlock product of ura3 deletion gRNA cassette Synthetic Ampicillin PMID:25281382 Backbone Size:6217; Vector Backbone:pRS42H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:16:22 5
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B
 
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RRID:Addgene_64221 Cas9 Streptococcus pyogenes Ampicillin PMID:25803306 Please note that due to the presence of BbsI sites in the Ad4 coding regions it is NOT possible to directly clone new sgRNA sequences into the BbsI sites downstream of the U6 promoter. Therefore, precloned U6-sgRNA cassettes, isolated as 1.7 kb PvuI-XbaI fragments from pU6-(BbsI)_CBh-Cas9-T2A-BFP (plasmid# 64323) or pU6-(BbsI)_CBh-Cas9-T2A-mCherry (plasmid# 64324) of this deposit, or any other plasmid derived from pX330 (plasmid# 42230), must be ligated into the PvuI-XbaI digested backbone of this Ad4 containing plasmid. Backbone Marker:Kuhn lab (Addgene plasmid # 64324); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-01 01:16:21 1
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6
 
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RRID:Addgene_64220 Cas9 Streptococcus pyogenes Ampicillin PMID:25803306 Please note that due to the presence of BbsI sites in the Ad4 coding regions it is NOT possible to directly clone new sgRNA sequences into the BbsI sites downstream of the U6 promoter. Therefore, precloned U6-sgRNA cassettes, isolated as 1.7 kb PvuI-XbaI fragments from pU6-(BbsI)_CBh-Cas9-T2A-BFP (plasmid# 64323) or pU6-(BbsI)_CBh-Cas9-T2A-mCherry (plasmid# 64324) of this deposit, or any other plasmid derived from pX330 (plasmid# 42230), must be ligated into the PvuI-XbaI digested backbone of this Ad4 containing plasmid. Backbone Marker:Kuhn lab (Addgene plasmid # 64323); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-BFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-01 01:16:34 0
pTet-IRES-EGFP
 
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RRID:Addgene_64238 Ampicillin PMID:25721206 The transgene of interest can be inserted into BamHI, PmeI, and PstI sites. However, only the PmeI and PstI sites are recommended to be used for different restriction end ligation. This plasmid must be used together with an independent tTA-expressing plasmid for Tet-Off system or rtTA-expressing plasmid for Tet-On system. The TetO promoter sequence was excised from pETE-Bsd plasmid vector, which was originated from pUHD-10-3. The pETE-Bsd and pUHD-10-13 were engineered by Protopopov et al. and Damke et al, respectively. Protopopov AI, Li J, Winberg G, Gizatullin RZ, Kashuba VI, Klein G, et al. Human cell lines engineered for tetracycline-regulated expression of tumor suppressor candidate genes from a frequently affected chromosomal region, 3p21. J Gene Med. 2002/07/19 ed. 2002;4(4):397–406. Damke, H., Gossen, M., Freundlieb, S., Bujard, H., and Schmid, S.L. (1995). Tightly regulated and inducible expression of dominant interfering dynamin mutant in stably transformed HeLa cells. Methods Enzymol. 257, 209–220. Backbone Marker:Didier Trono; Backbone Size:11101; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-01 01:16:34 5
pME-Cas9
 
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RRID:Addgene_64237 codon optimized Cas9 S. pyogenes Kanamycin PMID:25855067 Backbone Marker:Chi-Bin Chien lab; Backbone Size:2765; Vector Backbone:pME-MCS; Vector Types:CRISPR; Bacterial Resistance:Kanamycin 2026-08-01 01:16:22 2
pGGDestTol2LC-1sgRNA
 
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RRID:Addgene_64239 Chloramphenicol and Ampicillin PMID:25855067 Backbone Size:7083; Vector Backbone:pDestTol2LC; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-01 01:16:22 1
pMSCV-PIG-miR-146a
 
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RRID:Addgene_64234 miR146a Homo sapiens Ampicillin PMID:18066065 Backbone Marker:Scott Lowe; Backbone Size:7658; Vector Backbone:MSCV PIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-01 01:16:22 1
pU6d:sgRNA#5
 
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RRID:Addgene_64249 Spectinomycin PMID:25855067 Backbone Marker:Adam Bogdanove, Daniel Voytas lab; Backbone Size:2568; Vector Backbone:pLR-NN; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin 2026-08-01 01:16:22 1
pU6c:sgRNA#4
 
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RRID:Addgene_64248 Spectinomycin PMID:25855067 Backbone Marker:Adam Bogdanove, Daniel Voytas lab; Backbone Size:2568; Vector Backbone:pLR-NN; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin 2026-08-01 01:16:34 4
pGGDestTol2LC-2sgRNA
 
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RRID:Addgene_64240 Chloramphenicol and Ampicillin PMID:25855067 Backbone Size:7083; Vector Backbone:pDestTol2LC; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-01 01:16:22 1

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