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http://www.addgene.org/64294

Species: Synthetic
Genetic Insert: OmpA_H2467
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntFyLgLkMgIsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgLeLgVdVlgrmpykgsvengaykaqgVqLtAkLgYpitddVdVyLrLgImVwradtksnvygknhdtgvsPvFaGgVeYaItpeIaTrLeYqWtnnigdYPYDVPDYAtrpdnWmVsLgAsYrfgCCPGCCHHHHHH

Proper citation: RRID:Addgene_64294 Copy   

•   Source: Addgene

http://www.addgene.org/64293

Species: Synthetic
Genetic Insert: OmpA_H1248
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgLeLgVdVlgrmpykgsvengaykaqgVqLtAkLgYpitddVdVyLrLgImVwradtksnvygknhdtgvsFvMaLgSeHaItpeIaMrLeTqHtnnigdYPYDVPDYAtrpdnGmLsLgVsYrfgCCPGCCHHHHHH

Proper citation: RRID:Addgene_64293 Copy   

•   Source: Addgene

http://www.addgene.org/64211

Species: Rattus norvegicus
Genetic Insert: Kif5c
Vector Backbone Description: Backbone Marker:Banker lab (Addgene plasmid #45059); Backbone Size:8410; Vector Backbone:pBa.KIF5C 1-559.eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25624392
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination. XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.

Proper citation: RRID:Addgene_64211 Copy   

•   Source: Addgene

http://www.addgene.org/64332

Species: Synthetic
Genetic Insert: gBlock product of leu2 deletion gRNA cassette
Vector Backbone Description: Backbone Size:6217; Vector Backbone:pRS42H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25281382

Proper citation: RRID:Addgene_64332 Copy   

•   Source: Addgene

http://www.addgene.org/64214

Species: Arabidopsis thaliana
Genetic Insert: Cry2 PHR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24920824
Comments: PGK1 is cloned within the KpnI and XbaI sites

Proper citation: RRID:Addgene_64214 Copy   

•   Source: Addgene

http://www.addgene.org/64335

Vector Backbone Description: Backbone Marker:Theodora Agalioti; Vector Backbone:Migr1; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28508873
Comments: This construct was created in G. Stathopoulos lab in Patras, Greece, by Theodora Agalioti; The backbone is derived from MigR1 vector to which a partial IRES followed by puromycin selection gene derived from pPY_CAG_CRE_IP plasmid was inserted

Proper citation: RRID:Addgene_64335 Copy   

•   Source: Addgene

http://www.addgene.org/64210

Species: Mus musculus
Genetic Insert: Rab7
Vector Backbone Description: Backbone Size:6280; Vector Backbone:pBa-FRB-3myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25624392
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination. XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.

Proper citation: RRID:Addgene_64210 Copy   

•   Source: Addgene

http://www.addgene.org/64298

Species: Synthetic
Genetic Insert: OmpA_R4cons_G125L
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411

Proper citation: RRID:Addgene_64298 Copy   

•   Source: Addgene

http://www.addgene.org/64331

Species: Synthetic
Genetic Insert: gBlock product of trp1 deletion gRNA cassette
Vector Backbone Description: Backbone Size:6217; Vector Backbone:pRS42H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25281382
Comments: Note: Addgene's quality control sequencing finds one mismatch in the SNR52 promoter region, but plasmid function is not impacted.

Proper citation: RRID:Addgene_64331 Copy   

•   Source: Addgene

http://www.addgene.org/64297

Species: Synthetic
Genetic Insert: OmpA_H1358
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqQgIgAfAgIqVnpyVgFeMgYdWlgrmpykgsvengaykaqgYqAtLkMgVpitddLdIyTrLgGmVwradtksnvygknhdtgvsFvMaLgSeHaItpeIaMrLeTqHtnnigdYPYDVPDYAtrpdnGmLsLgVsYrfgCCPGCCHHHHHH

Proper citation: RRID:Addgene_64297 Copy   

•   Source: Addgene

http://www.addgene.org/64330

Species: Synthetic
Genetic Insert: gBlock product of ura3 deletion gRNA cassette
Vector Backbone Description: Backbone Size:6217; Vector Backbone:pRS42H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25281382

Proper citation: RRID:Addgene_64330 Copy   

•   Source: Addgene

http://www.addgene.org/64221

Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kuhn lab (Addgene plasmid # 64324); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25803306
Comments: Please note that due to the presence of BbsI sites in the Ad4 coding regions it is NOT possible to directly clone new sgRNA sequences into the BbsI sites downstream of the U6 promoter. Therefore, precloned U6-sgRNA cassettes, isolated as 1.7 kb PvuI-XbaI fragments from pU6-(BbsI)_CBh-Cas9-T2A-BFP (plasmid# 64323) or pU6-(BbsI)_CBh-Cas9-T2A-mCherry (plasmid# 64324) of this deposit, or any other plasmid derived from pX330 (plasmid# 42230), must be ligated into the PvuI-XbaI digested backbone of this Ad4 containing plasmid.

Proper citation: RRID:Addgene_64221 Copy   

•   Source: Addgene

http://www.addgene.org/64220

Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kuhn lab (Addgene plasmid # 64323); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-BFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25803306
Comments: Please note that due to the presence of BbsI sites in the Ad4 coding regions it is NOT possible to directly clone new sgRNA sequences into the BbsI sites downstream of the U6 promoter. Therefore, precloned U6-sgRNA cassettes, isolated as 1.7 kb PvuI-XbaI fragments from pU6-(BbsI)_CBh-Cas9-T2A-BFP (plasmid# 64323) or pU6-(BbsI)_CBh-Cas9-T2A-mCherry (plasmid# 64324) of this deposit, or any other plasmid derived from pX330 (plasmid# 42230), must be ligated into the PvuI-XbaI digested backbone of this Ad4 containing plasmid.

Proper citation: RRID:Addgene_64220 Copy   

•   Source: Addgene

http://www.addgene.org/64238

Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11101; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25721206
Comments: The transgene of interest can be inserted into BamHI, PmeI, and PstI sites. However, only the PmeI and PstI sites are recommended to be used for different restriction end ligation. This plasmid must be used together with an independent tTA-expressing plasmid for Tet-Off system or rtTA-expressing plasmid for Tet-On system. The TetO promoter sequence was excised from pETE-Bsd plasmid vector, which was originated from pUHD-10-3. The pETE-Bsd and pUHD-10-13 were engineered by Protopopov et al. and Damke et al, respectively. Protopopov AI, Li J, Winberg G, Gizatullin RZ, Kashuba VI, Klein G, et al. Human cell lines engineered for tetracycline-regulated expression of tumor suppressor candidate genes from a frequently affected chromosomal region, 3p21. J Gene Med. 2002/07/19 ed. 2002;4(4):397–406. Damke, H., Gossen, M., Freundlieb, S., Bujard, H., and Schmid, S.L. (1995). Tightly regulated and inducible expression of dominant interfering dynamin mutant in stably transformed HeLa cells. Methods Enzymol. 257, 209–220.

Proper citation: RRID:Addgene_64238 Copy   

•   Source: Addgene

http://www.addgene.org/64237

Species: S. pyogenes
Genetic Insert: codon optimized Cas9
Vector Backbone Description: Backbone Marker:Chi-Bin Chien lab; Backbone Size:2765; Vector Backbone:pME-MCS; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25855067

Proper citation: RRID:Addgene_64237 Copy   

•   Source: Addgene

http://www.addgene.org/64239

Vector Backbone Description: Backbone Size:7083; Vector Backbone:pDestTol2LC; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25855067

Proper citation: RRID:Addgene_64239 Copy   

•   Source: Addgene

http://www.addgene.org/64234

Species: Homo sapiens
Genetic Insert: miR146a
Vector Backbone Description: Backbone Marker:Scott Lowe; Backbone Size:7658; Vector Backbone:MSCV PIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18066065

Proper citation: RRID:Addgene_64234 Copy   

•   Source: Addgene

http://www.addgene.org/64249

Vector Backbone Description: Backbone Marker:Adam Bogdanove, Daniel Voytas lab; Backbone Size:2568; Vector Backbone:pLR-NN; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25855067

Proper citation: RRID:Addgene_64249 Copy   

•   Source: Addgene

http://www.addgene.org/64248

Vector Backbone Description: Backbone Marker:Adam Bogdanove, Daniel Voytas lab; Backbone Size:2568; Vector Backbone:pLR-NN; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25855067

Proper citation: RRID:Addgene_64248 Copy   

•   Source: Addgene

http://www.addgene.org/64240

Vector Backbone Description: Backbone Size:7083; Vector Backbone:pDestTol2LC; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25855067

Proper citation: RRID:Addgene_64240 Copy   

•   Source: Addgene