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Species: Homo sapiens
Genetic Insert: sgRNA2_NANOG
Vector Backbone Description: Backbone Marker:Stanley Qi, Addgene plasmid 44248; Vector Backbone:pgRNA-humanized; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25619936
Proper citation: RRID:Addgene_64154 Copy
Species: Mus musculus
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:6280; Vector Backbone:pBa-FRB-3myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25624392
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_64209 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24920824
Proper citation: RRID:Addgene_64208 Copy
Genetic Insert: Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
Vector Backbone Description: Backbone Marker:George Church lab (Addgene Plasmid #43802); Backbone Size:9232; Vector Backbone:pRS414-TEF1p-Cas9-CYC1t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25281382
Proper citation: RRID:Addgene_64329 Copy
Species: Synthetic
Genetic Insert: CrBlue
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23479634
Comments: CrBlue was cloned into the EcoR1 and NotI sites of pcDNA3.1 and mcherry was fused to the C terminus of CrBlue using the NotI and XbaI sites.
Proper citation: RRID:Addgene_64204 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24920824
Proper citation: RRID:Addgene_64207 Copy
Species: Mus musculus
Genetic Insert: Bicaudal D protein
Vector Backbone Description: Backbone Size:8145; Vector Backbone:pBa-tdTomato-flag-BicD2 594-FKBP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25624392
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_64206 Copy
Species: Homo sapiens
Genetic Insert: Nup88
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21289091
Proper citation: RRID:Addgene_64283 Copy
Species: Mus musculus
Genetic Insert: KIF13B
Vector Backbone Description: Backbone Size:6287; Vector Backbone:pBa-FRB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25624392
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_64289 Copy
Species: Aequorea victoria
Genetic Insert: Venus
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Artificial targeting vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25803306
Proper citation: RRID:Addgene_64322 Copy
Species: Drosophila melanogaster
Genetic Insert: Wingless
Vector Backbone Description: Backbone Size:12500; Vector Backbone:pCaSpeR4; Vector Types:Drosophila Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18430724
Proper citation: RRID:Addgene_64321 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid # 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25803306
Proper citation: RRID:Addgene_64324 Copy
Species: Mus musculus
Genetic Insert: KIF1B beta
Vector Backbone Description: Backbone Size:6287; Vector Backbone:pBa-FRB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25624392
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_64287 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kuhn lab (Addgene plasmid # 64218); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25803306
Proper citation: RRID:Addgene_64219 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:6691; Vector Backbone:6691 Bicistronic_ires_puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28508873
Comments: This construct was created in G. Stahopoulos lab in Patras, by Theodora Agalioti and represents a retroviral expression vector of Green Fluorescent protein in a bicistronic fashion along with puromycin resistance gene
Proper citation: RRID:Addgene_64336 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kuhn lab (Addgene plasmid # 64323); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-BFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25803306
Comments: Please note that due to the presence of BbsI sites in the Ad4 coding regions it is NOT possible to directly clone new sgRNA sequences into the BbsI sites downstream of the U6 promoter.
Therefore, precloned U6-sgRNA cassettes, isolated as 1.7 kb PvuI-XbaI fragments from pU6-(BbsI)_CBh-Cas9-T2A-BFP (plasmid# 64323) or pU6-(BbsI)_CBh-Cas9-T2A-mCherry (plasmid# 64324) of this deposit, or any other plasmid derived from pX330 (plasmid# 42230), must be ligated into the PvuI-XbaI digested backbone of this Ad4 containing plasmid.
Proper citation: RRID:Addgene_64218 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kuhn lab (Addgene plasmid # 64323); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-BFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25803306
Proper citation: RRID:Addgene_64217 Copy
Species: Homo sapiens
Genetic Insert: Centrin1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIREShyg3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_64338 Copy
Species: Synthetic
Genetic Insert: OmpA_H3478
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntFyLgLkMgIsqyhdtgfinnngpthenqQgIgAfAgIqVnpyVgFeMgYdWlgrmpykgsvengaykaqgVqLtAkLgYpitddVdVyLrLgImVwradtksnvygknhdtgvsFvMaLgSeHaItpeIaTrLeYqWtnnigdYPYDVPDYAtrpdnGmLsLgVsYrfgCCPGCCHHHHHH
Proper citation: RRID:Addgene_64292 Copy
Species: Synthetic
Genetic Insert: OmpA_H2578
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntFyLgLkMgIsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgLeLgVdVlgrmpykgsvengaykaqgYqAtLkMgVpitddLdIyTrLgGmVwradtksnvygknhdtgvsFvMaLgSeHaItpeIaTrLeYqWtnnigdYPYDVPDYAtrpdnGmLsLgVsYrfgCCPGCCHHHHHH
Proper citation: RRID:Addgene_64291 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
