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On page 147 showing 2921 ~ 2940 out of 739,423 results
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    • RRID:Addgene_170631

http://www.addgene.org/170631

Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:34152213
Comments:

Proper citation: RRID:Addgene_170631 Copy   

http://www.addgene.org/170753

Species: Gallus gallus
Genetic Insert: PLA2G4A-C2 domain
Vector Backbone Description: Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31050338
Comments:

Proper citation: RRID:Addgene_170753 Copy   

    • RRID:Addgene_170637

http://www.addgene.org/170637

Species: Saccharomyces cerevisiae
Genetic Insert: Mini-Tn6677, GNAc cassette, lacI
Vector Backbone Description: Vector Backbone:pUC57mini; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34152213
Comments:

Proper citation: RRID:Addgene_170637 Copy   

http://www.addgene.org/170625

Species: Synthetic
Genetic Insert: Lyn11-myc-LOV2GIVe (D)
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pLIC-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32936073
Comments:

Proper citation: RRID:Addgene_170625 Copy   

    • RRID:Addgene_170744

http://www.addgene.org/170744

Species: Homo sapiens
Genetic Insert: CPTP
Vector Backbone Description: Vector Backbone:pFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23863933
Comments:

Proper citation: RRID:Addgene_170744 Copy   

    • RRID:Addgene_170742

http://www.addgene.org/170742

Species: Homo sapiens
Genetic Insert: CPTP
Vector Backbone Description: Vector Backbone:pFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21625605
Comments:

Proper citation: RRID:Addgene_170742 Copy   

    • RRID:Addgene_170740

http://www.addgene.org/170740

Species: Homo sapiens
Genetic Insert: GLTP
Vector Backbone Description: Vector Backbone:pFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21625605
Comments:

Proper citation: RRID:Addgene_170740 Copy   

    • RRID:Addgene_170629

http://www.addgene.org/170629

Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:34152213
Comments:

Proper citation: RRID:Addgene_170629 Copy   

http://www.addgene.org/17072

Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Description: Zebrafish prickle-1 (full-length ORF), cloned into the BamH1 and EcoR1 sites of CS2P+. Species of Sequence Origin: zebrafish. Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp. Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).

Proper citation: RRID:Addgene_17072 Copy   

http://www.addgene.org/17074

Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:CS2+GFP-BB; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP. The C-terminal prenylation motif KNCIIS was deleted by PCR. Species of Sequence Origin: zebrafish. Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp. Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).

Proper citation: RRID:Addgene_17074 Copy   

    • RRID:Addgene_17064

http://www.addgene.org/17064

Species: Danio rerio
Genetic Insert: vox-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:
Comments: Zvox-2 coding region amplified by PCR and inserted into CS2+ (first cloned into SK as zV93). For RNA: cut with Asp718 and transcribe with SP6. Plasmid History: Species of Sequence Origin: Zebrafish. Constructed by: David Kimelman, 6/28/97 XIX p. 67.

Proper citation: RRID:Addgene_17064 Copy   

http://www.addgene.org/17068

Species: Danio rerio
Genetic Insert: Stbm
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780127
Comments: 6-c-myc epitopes in frame at the amino terminus of wild type zebrafish strabismus; EcoRI-Xba1 insert. For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002.

Proper citation: RRID:Addgene_17068 Copy   

http://www.addgene.org/170810

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:lentiCRISPR v2 (Addgene #52961); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34133940
Comments: 3rd generation lentiviral backbone. Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint.

Proper citation: RRID:Addgene_170810 Copy   

    • RRID:Addgene_170774

    This resource has 1+ mentions.

http://www.addgene.org/170774

Species: Homo sapiens
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Marker:Addgene (Plasmid #14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34102054
Comments: Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318

Proper citation: RRID:Addgene_170774 Copy   

    • RRID:Addgene_170775

    This resource has 1+ mentions.

http://www.addgene.org/170775

Species: Homo sapiens
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Marker:Addgene Plasmid #19319; Backbone Size:8083; Vector Backbone:pLJM1-EGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34102054
Comments: Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318

Proper citation: RRID:Addgene_170775 Copy   

http://www.addgene.org/170772

Species: Synthetic
Genetic Insert: lacI
Vector Backbone Description: Backbone Marker:pTwist; Backbone Size:3; Vector Backbone:pUC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35255734
Comments: This plasmid was designed to test Golden Gate assembly protocols

Proper citation: RRID:Addgene_170772 Copy   

http://www.addgene.org/170818

Species: Homo sapiens
Genetic Insert: PCNA Homology Arms with mEGFP-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human PCNA in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GEGQGQGQGPGRGYAYRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .

Proper citation: RRID:Addgene_170818 Copy   

http://www.addgene.org/170813

Species: Synthetic; B. coriaceae
Genetic Insert: NLS-mScarlet-I-P2A-BlastR
Vector Backbone Description: Vector Backbone:pW212 (Addgene #170810); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34133940
Comments: 3rd generation lentiviral backbone. Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint.

Proper citation: RRID:Addgene_170813 Copy   

http://www.addgene.org/170770

Species: Synthetic
Genetic Insert: lacI
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:1835; Vector Backbone:pUC; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35255734
Comments: This plasmid was designed to test 5RM assembly protocols

Proper citation: RRID:Addgene_170770 Copy   

http://www.addgene.org/170771

Species: Synthetic
Genetic Insert: tagRFP
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:2579; Vector Backbone:pUC; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35255734
Comments: This plasmid was designed to test Golden Gate assembly protocols

Proper citation: RRID:Addgene_170771 Copy   

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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."

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