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http://www.addgene.org/170583

Species: Homo sapiens
Genetic Insert: miniTurbo-V5-MARK3
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.

Proper citation: RRID:Addgene_170583 Copy   

•   Source: Addgene

http://www.addgene.org/170584

Species: Homo sapiens
Genetic Insert: miniTurbo-V5-PSKH1
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.

Proper citation: RRID:Addgene_170584 Copy   

•   Source: Addgene

http://www.addgene.org/170581

Species: Homo sapiens
Genetic Insert: miniTurbo-V5-MAPK15
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.

Proper citation: RRID:Addgene_170581 Copy   

•   Source: Addgene

http://www.addgene.org/170580

Species: Homo sapiens
Genetic Insert: miniTurbo-V5-LMTK2
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.

Proper citation: RRID:Addgene_170580 Copy   

•   Source: Addgene

http://www.addgene.org/170537

Species: Homo sapiens
Genetic Insert: Protein tyrosine phosphatase interacting protein 51
Vector Backbone Description: Backbone Size:6034; Vector Backbone:pCAG_FLAG_puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33938112
Comments: Note that deletion is of amino acids 151-175.

Proper citation: RRID:Addgene_170537 Copy   

•   Source: Addgene

http://www.addgene.org/170534

Species: Homo sapiens
Genetic Insert: Protein tyrosine phosphatase interacting protein 51
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5537; Vector Backbone:pQlinkG2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33938112

Proper citation: RRID:Addgene_170534 Copy   

•   Source: Addgene

http://www.addgene.org/170533

Species: Homo sapiens
Genetic Insert: Protein tyrosine phosphatase interacting protein 51
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:4901; Vector Backbone:pQlinkH; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33938112

Proper citation: RRID:Addgene_170533 Copy   

•   Source: Addgene

http://www.addgene.org/170491

Species: Homo sapiens
Genetic Insert: mCherry, ITSN(N-lobe), iFKBP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279442
Comments: The plasmid includes a 4 amino acid -GS linker (SGGS) downstream of mCherry that is not included in the original uploaded sequence.

Proper citation: RRID:Addgene_170491 Copy   

•   Source: Addgene

http://www.addgene.org/170490

Species: Tobacco Etch Virus (TEV)
Genetic Insert: myc, FRB, TEV(C-lobe)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279442

Proper citation: RRID:Addgene_170490 Copy   

•   Source: Addgene

http://www.addgene.org/170526

Species: Mus musculus
Genetic Insert: Caveolin1
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:leishmania cell free expression clone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33904409

Proper citation: RRID:Addgene_170526 Copy   

•   Source: Addgene

http://www.addgene.org/170523

Species: Mus musculus
Genetic Insert: Cavin4
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33904409

Proper citation: RRID:Addgene_170523 Copy   

•   Source: Addgene

http://www.addgene.org/170524

Species: Mus musculus
Genetic Insert: Cavin4
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33904409

Proper citation: RRID:Addgene_170524 Copy   

•   Source: Addgene

http://www.addgene.org/170489

Species: Tobacco Etch Virus (TEV)
Genetic Insert: TEV(N-lobe), iFKBP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279442

Proper citation: RRID:Addgene_170489 Copy   

•   Source: Addgene

http://www.addgene.org/170486

Species: Homo sapiens
Genetic Insert: GDI1(N-lobe)-iFKBP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279442

Proper citation: RRID:Addgene_170486 Copy   

•   Source: Addgene

http://www.addgene.org/170487

Species: Homo sapiens
Genetic Insert: FRB-GDI1(C-lobe)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279442

Proper citation: RRID:Addgene_170487 Copy   

•   Source: Addgene

http://www.addgene.org/170520

Species: Synthetic
Genetic Insert: CAG-loxP-STOP-loxP-Venus
Vector Backbone Description: Vector Backbone:pXL-BacII; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34458399
Comments: More information can be found in this additional reference: Yamagata M, Yan W, Sanes JR. A cell atlas of the chick retina based on single-cell transcriptomics. Elife. 2021 Jan 4;10:e63907. doi: 10.7554/eLife.63907. PMID: 33393903; PMCID: PMC7837701.

Proper citation: RRID:Addgene_170520 Copy   

•   Source: Addgene

http://www.addgene.org/17050

Species: Danio rerio
Genetic Insert: wnt8
Vector Backbone Description: Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:; Bacterial Resistance:Ampicillin
Comments: ORFs ligated into BamHI, ClaI cut pCS2+MT. Adds 6 myc tags onto carboxy terminus of each. For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish.

Proper citation: RRID:Addgene_17050 Copy   

•   Source: Addgene

http://www.addgene.org/170527

Species: Mus musculus
Genetic Insert: Caveolin1
Vector Backbone Description: Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33904409

Proper citation: RRID:Addgene_170527 Copy   

•   Source: Addgene

http://www.addgene.org/17052

Species: Danio rerio
Genetic Insert: wnt8 ORF1/2
Vector Backbone Description: Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:; Bacterial Resistance:Ampicillin
Comments: wnt8 ORFs + IREs cloned into BamHI-ClaI sites of pCS2+MT. Adds 6 myc tags onto c-terminus of ORF2. For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish.

Proper citation: RRID:Addgene_17052 Copy   

•   Source: Addgene

http://www.addgene.org/17048

Species: Danio rerio
Genetic Insert: wnt8
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+ (modified pCS2+); Vector Types:; Bacterial Resistance:Ampicillin
Comments: ORF1 amplified from pBand 3-5 (full length cDNA clone) ligated into BamHI-StuI cut CS2P+. For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish.

Proper citation: RRID:Addgene_17048 Copy   

•   Source: Addgene