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Species: Synthetic
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167881 Copy
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167924 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene Plasmid #52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33622708
Proper citation: RRID:Addgene_167929 Copy
Genetic Insert: T2A-EGFP
Vector Backbone Description: Backbone Marker:Eric Lander, David Sabatini (Addgene Plasmid #50661); Backbone Size:11885; Vector Backbone:pCW-Cas9; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33622708
Proper citation: RRID:Addgene_167928 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167873 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167876 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167875 Copy
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167915 Copy
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167914 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167879 Copy
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167919 Copy
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167917 Copy
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167916 Copy
Species: Synthetic
Genetic Insert: EKAREN4
Vector Backbone Description: Backbone Size:3450; Vector Backbone:miniTol2 expression vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33795873
Comments: We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis. The generation of stable cell lines using this plasmid requires the co-transfection of a transposase-expressing plasmid. The depositing lab recommends the use of Plasmid 158774.
Proper citation: RRID:Addgene_167824 Copy
Species: Synthetic
Genetic Insert: EKAREN4
Vector Backbone Description: Vector Backbone:pLV lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33795873
Comments: We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis.
Proper citation: RRID:Addgene_167829 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB-MeCP2
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167896 Copy
Species: Synthetic
Genetic Insert: Cas9-VPR
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167895 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene Plasmid #52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33622708
Proper citation: RRID:Addgene_167932 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene Plasmid #52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33622708
Proper citation: RRID:Addgene_167930 Copy
Species: Synthetic
Genetic Insert: Cas9-VPR
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167892 Copy
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