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Species: Homo sapiens
Genetic Insert: IKBKB
Vector Backbone Description: Backbone Size:9514; Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34646987
Proper citation: RRID:Addgene_170226 Copy
Species: Homo sapiens
Genetic Insert: CDK6
Vector Backbone Description: Backbone Size:9514; Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34646987
Proper citation: RRID:Addgene_170224 Copy
Species: Homo sapiens
Genetic Insert: ER
Vector Backbone Description: Backbone Size:9514; Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34646987
Proper citation: RRID:Addgene_170225 Copy
Species: Homo sapiens
Genetic Insert: CDK4
Vector Backbone Description: Backbone Size:9514; Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34646987
Proper citation: RRID:Addgene_170222 Copy
Species: Homo sapiens
Genetic Insert: CDK1
Vector Backbone Description: Backbone Size:9514; Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34646987
Proper citation: RRID:Addgene_170220 Copy
Species: Homo sapiens
Genetic Insert: CDK1
Vector Backbone Description: Backbone Size:9514; Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34646987
Proper citation: RRID:Addgene_170221 Copy
Species: Escherichia coli
Genetic Insert: Ec86 RT
Vector Backbone Description: Backbone Marker:Fraser lab; Vector Backbone:pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35076284
Proper citation: RRID:Addgene_170185 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:9514; Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34646987
Proper citation: RRID:Addgene_170248 Copy
Species: Tetrahymena thermophila
Genetic Insert: Q22YU3 C-terminal truncation (Delta-C3)
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33632841
Proper citation: RRID:Addgene_170316 Copy
Species: HIV-2
Genetic Insert: HIV-2 RT p66 (mutant)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3498; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:34082799
Comments: Please visit https://www.medrxiv.org/content/10.1101/2020.08.13.20173757v4 for medRxiv preprint
Proper citation: RRID:Addgene_170279 Copy
Species: Bacillus stearothermophilus
Genetic Insert: Bst-LF D720A
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3543; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:34082799
Comments: Please visit https://www.medrxiv.org/content/10.1101/2020.08.13.20173757v4 for medRxiv preprint
Proper citation: RRID:Addgene_170277 Copy
Species: Tetrahymena thermophila
Genetic Insert: Outer Dynein Arm Intermediate Chain 3
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33632841
Comments: Reference for source of plasmid backbone:
Kataoka, K., Schoeberl, U.E., and Mochizuki, K. (2010). Modules for C-terminal epitope tagging of Tetrahymena genes. J. Microbiol. Methods 82, 342–346.
Proper citation: RRID:Addgene_170311 Copy
Species: Homo sapiens
Genetic Insert: RPL22
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32516938
Proper citation: RRID:Addgene_170319 Copy
Species: Bradyrhizobium sp. (strain ORS 278)
Genetic Insert: NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
Vector Backbone Description: Backbone Marker:Originally derived from pEGFP-N1 backbone after several modifications in our lab.; Backbone Size:3427; Vector Backbone:pQP-Select; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34162879
Comments: To design iLight, a full-length bacterial phytochrome IsPadC from Idiomarina sp. A28L was used as an original template. First, diguanylyl cyclase effector in IsPadC was removed, then following mutations were introduced: I68F, H80Q, A86T, R90S S242C, E274K, R295H I360V, and L464V. After that, iLight was human codon optimized for mammalian expression.
Proper citation: RRID:Addgene_170273 Copy
Species: Homo sapiens
Genetic Insert: SEAP
Vector Backbone Description: Backbone Marker:GeneScript; Backbone Size:4108; Vector Backbone:pG12; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34162879
Proper citation: RRID:Addgene_170271 Copy
Species: Renilla reniformis
Genetic Insert: RLuc8
Vector Backbone Description: Backbone Marker:GeneScript; Backbone Size:4108; Vector Backbone:pG12; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34162879
Proper citation: RRID:Addgene_170272 Copy
Species: Idiomarina sp. A28L
Genetic Insert: iLight
Vector Backbone Description: Backbone Marker:Life Technologies-Invitrogen (partially modified); Backbone Size:3999; Vector Backbone:Modified pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34162879
Comments: To design iLight for expression in bacteria, a full-length bacterial phytochrome IsPadC from Idiomarina sp. A28L was used as an original template. First, diguanylyl cyclase effector in IsPadC was removed, then following mutations were introduced: I68F, H80Q, A86T, R90S S242C, E274K, R295H I360V, and L464V.
Proper citation: RRID:Addgene_170270 Copy
Genetic Insert: A gRNA targeting the mouse Tbxas1 gene.
Vector Backbone Description: Backbone Marker:https://www.addgene.org/52961/; Vector Backbone:LentiCRISPRv2puro; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34035084
Proper citation: RRID:Addgene_170305 Copy
Genetic Insert: A gRNA targeting the mouse Ptger1 gene.
Vector Backbone Description: Backbone Marker:https://www.addgene.org/52961/; Vector Backbone:LentiCRISPRv2puro; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34035084
Proper citation: RRID:Addgene_170306 Copy
Genetic Insert: A gRNA targeting the mouse Ptgfr gene.
Vector Backbone Description: Backbone Marker:https://www.addgene.org/52961/; Vector Backbone:LentiCRISPRv2puro; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34035084
Proper citation: RRID:Addgene_170303 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
