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Species: Synthetic
Genetic Insert: bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
Vector Backbone Description: Backbone Size:5238; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
References:
Comments: This is a bacterial codon-optimized SpCas9 (H840A) sequence within pET-28b backbone (Addgene #73018) fused to bipartite NLS, a 33-amino acid linker and the engineered M-MLV RT from the parental pCMV-PE2 plasmid (Addgene #132775). To this construct, a short GS linker and a 6xHis-tag are added to C terminus.
Proper citation: RRID:Addgene_170103 Copy
Species:
Genetic Insert: T7-RNA polymerase
Vector Backbone Description: Backbone Size:3262; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_170101 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170100 Copy
Species:
Genetic Insert: sgBFP
Vector Backbone Description: Backbone Marker:Fraser lab; Vector Backbone:pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170183 Copy
Species: Stigmatella aurantica
Genetic Insert: Sa163 RT
Vector Backbone Description: Backbone Marker:Fraser lab; Vector Backbone:pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170184 Copy
Species: Homo sapiens
Genetic Insert: ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_170136 Copy
Species: E. coli
Genetic Insert: E. coli type I-E Cas5
Vector Backbone Description: Backbone Size:5221; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: If used in a publication, please acknowledge that the plasmid pEcCas5 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170090 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170099 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170097 Copy
Species: Mus musculus
Genetic Insert: nCas9-SpG(H840A)-M-MLV
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170130 Copy
Species: Mus musculus
Genetic Insert: nCas9(H840A)-M-MLV
Vector Backbone Description: Backbone Marker:Qi-Jun Chen; Vector Backbone:pHUE411 (modified from pCambia); Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_170131 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170096 Copy
Species: E. coli
Genetic Insert: E. coli type I-E Cse2
Vector Backbone Description: Backbone Size:5230; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: If used in a publication, please acknowledge that the plasmid pEcCse2 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170094 Copy
Species: Homo sapiens
Genetic Insert: MS2-ADAR2(3'UAG)-MS2
Vector Backbone Description: Backbone Marker:Addgene #52961; Backbone Size:10500; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170128 Copy
Species: Homo sapiens
Genetic Insert: MS2-ADAR2(5'UAG)-MS2
Vector Backbone Description: Backbone Marker:Addgene #52961; Backbone Size:10500; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170126 Copy
Species: Bacteria
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Backbone Size:2076; Vector Backbone:pZa; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_170081 Copy
Species:
Genetic Insert: E. coli type I-E single spacer array
Vector Backbone Description: Backbone Size:1688; Vector Backbone:ColE1; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_170088 Copy
Species: Homo sapiens
Genetic Insert: Circular 200,100 guide RNA with 8bp bulge loops
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_170121 Copy
Species: Mus musculus
Genetic Insert: Circular 200,100 guide RNA
Vector Backbone Description: Backbone Marker:Addgene #78601; Backbone Size:5700; Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170122 Copy
Species: Bacteria
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Backbone Size:2078; Vector Backbone:pZa; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_170084 Copy
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