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Species:
Genetic Insert: SauCas9 EMX1-nicking sgRNA
Vector Backbone Description: Backbone Size:600; Vector Backbone:pBlueScript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169860 Copy
Species:
Genetic Insert: Spy EMX1-pegRNA
Vector Backbone Description: Backbone Size:600; Vector Backbone:pBlueScript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169855 Copy
Species:
Genetic Insert: SaCas9KKH-N580A-M-MLV
Vector Backbone Description: Backbone Size:5517; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169852 Copy
Species:
Genetic Insert: SpCas9-H840A-star-M-MLV-3HA
Vector Backbone Description: Backbone Size:6635; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169854 Copy
Species:
Genetic Insert: spCas9 PE*
Vector Backbone Description: Backbone Size:3400; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169850 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: U6 U57A
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone.
Proper citation: RRID:Addgene_169927 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: U6 U57C
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone.
Proper citation: RRID:Addgene_169928 Copy
Species: Homo sapiens
Genetic Insert: kinesin family member 5B, isoform CRA_a
Vector Backbone Description: Backbone Marker:Novagene; Backbone Size:3306; Vector Backbone:pET-17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169995 Copy
Species: Homo sapiens
Genetic Insert: Kinesin-like protein Unc-104 fused with KIF5B
Vector Backbone Description: Backbone Marker:Novagene; Backbone Size:3306; Vector Backbone:pET-17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169996 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009).
Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170019 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169940 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009).
Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170016 Copy
Species:
Genetic Insert: guide RNAs targeting E1, E2, and E3 ubiquitin ligases, deubiquitinases, and control genes
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169942 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009).
Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170014 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009).
Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170012 Copy
Species: Homo sapiens
Genetic Insert: DPP9
Vector Backbone Description: Vector Backbone:pFastBac HTB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169947 Copy
Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169949 Copy
Species: Homo sapiens
Genetic Insert: BCL6 1-275AA
Vector Backbone Description: Vector Backbone:Cilantro 2 Plasmid #74450; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: https://benchling.com/s/seq-NeFKwxNgn6G0YAMn9Ym6
Proper citation: RRID:Addgene_169932 Copy
Species: Homo sapiens
Genetic Insert: BCL6 R28A
Vector Backbone Description: Vector Backbone:Artichoke (Plasmid #73320); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: https://benchling.com/s/seq-iwKB5ThUKLezYuBPsi9T
Proper citation: RRID:Addgene_169933 Copy
Species: Aequorea victoria
Genetic Insert: Superfolder GFP
Vector Backbone Description: Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170009 Copy
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