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Species: Homo sapiens
Genetic Insert: TFRC
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pMXS-IRES-BLAST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169890 Copy
Species: Homo sapiens
Genetic Insert: tCD19
Vector Backbone Description: Vector Backbone:PX458; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.22.427783v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_169817 Copy
Species: Mus musculus
Genetic Insert: pegCTNNB1 S45DEL
Vector Backbone Description: Vector Backbone:pmd127; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169844 Copy
Species: Mus musculus
Genetic Insert: sgCTNNB1 Nicking
Vector Backbone Description: Vector Backbone:pmd127; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169845 Copy
Species: Homo sapiens
Genetic Insert: sgAAT Nicking
Vector Backbone Description: Vector Backbone:pmd127; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169842 Copy
Species: SARS coronavirus 2
Genetic Insert: Protein S
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10381; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169848 Copy
Species: SARS coronavirus 2
Genetic Insert: Protein S
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10381; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169849 Copy
Species: Xenopus laevis
Genetic Insert: Goosecoid
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: coding region of Xenopus goosecoid in SP64T in sense (#9) & antisense (#2) orientation.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian by PCR.
Goosecoid cloned and characterized by lab of E. DeRobertis, UCLA.
Proper citation: RRID:Addgene_16979 Copy
Species: Mus musculus
Genetic Insert: safe harbor sgRNA
Vector Backbone Description: Backbone Size:8907; Vector Backbone:pMCB320; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169834 Copy
Species: Xenopus laevis
Genetic Insert: Xbra
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:psp73; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: full length Xbra from Jim Smith.
Antisense: linearize with EcoRV, transcribe with T7.
Plasmid History:
Constructed by Jan Christian.
Proper citation: RRID:Addgene_16978 Copy
Species: Homo sapiens
Genetic Insert: guide RNA for KLF7 neighbouring region
Vector Backbone Description: Backbone Marker:Addgene Plasmid #52961; Backbone Size:12993; Vector Backbone:LentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169797 Copy
Species: Homo sapiens
Genetic Insert: Donor sequence to ACTB gene for HR
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2959; Vector Backbone:pBlueScript II SK (+); Vector Types:HR donor vector for human ACTB gene.; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169798 Copy
Species: Synthetic
Genetic Insert: OxLight1
Vector Backbone Description: Backbone Marker:Viral Vector Facility - University of Zurich; Backbone Size:4438; Vector Backbone:pAAV.hSynapsin1; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169792 Copy
Species:
Genetic Insert: Scrambled guide RNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #52961; Backbone Size:12993; Vector Backbone:LentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169795 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: FnCas12a
Vector Backbone Description: Backbone Size:4447; Vector Backbone:pNW33n; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_169838 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:8535; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169745 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:5422; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169746 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6529; Vector Backbone:pPIDCB2; Vector Types:Mammalian Expression, Avian expression; Bacterial Resistance:Ampicillin
References:
Comments: Use AflII and PstI restriction sites for cloning. This plasmid is identical to pPIDNB2 except it has replaced the mNeonGreen sequence in pPIDNB2, which requires a paid Allele Biotech license, with mClover3, which doesn't require a paid license.
Proper citation: RRID:Addgene_169900 Copy
Species: Synthetic
Genetic Insert: tetR-P2A-eGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6113; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Note that although the plasmid encodes a hygromycin resistance gene, it is not expressed from the plasmid. It is only expressed after Flp recombinase-mediated chromosomal integration of the plasmid.
Proper citation: RRID:Addgene_169747 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6916; Vector Backbone:pPIDNB2; Vector Types:Mammalian Expression, Avian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Use AflII and PstI restriction sites for cloning. This plasmid contains mNeonGreen, which requires a paid license. See pPIDCB2 for an alternative plasmid, which has replaced the mNeongreen with mClover3 and does not require a paid license.
Proper citation: RRID:Addgene_169902 Copy
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