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http://www.addgene.org/34683

Species: Homo sapiens
Genetic Insert: dynamin 1ba
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_34683 Copy   

•   Source: Addgene

http://www.addgene.org/34688

Species: Homo sapiens
Genetic Insert: dynamin 1
Vector Backbone Description: Vector Backbone:pUHD10-3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_34688 Copy   

•   Source: Addgene

http://www.addgene.org/34841

Species: Mus musculus
Genetic Insert: FKBP-LAP1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pcDNA4; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_34841 Copy   

•   Source: Addgene

http://www.addgene.org/34682

Species: Homo sapiens
Genetic Insert: dynamin 1ba
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_34682 Copy   

•   Source: Addgene

http://www.addgene.org/34681

Species: Homo sapiens
Genetic Insert: dynamin 1ba
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_34681 Copy   

•   Source: Addgene

http://www.addgene.org/34796

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:Derived from pBluescript II SK+; Vector Types:Hydra Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_34796 Copy   

•   Source: Addgene

http://www.addgene.org/34794

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:Derived from pBluescript II SK+; Vector Types:Hydra Expression; Bacterial Resistance:Ampicillin
References:
Comments: https://www.ncbi.nlm.nih.gov/nuccore/158426709

Proper citation: RRID:Addgene_34794 Copy   

•   Source: Addgene

http://www.addgene.org/34712

Species: Mus musculus
Genetic Insert: Hoxd10
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:in situ hybridization probe; Bacterial Resistance:Ampicillin
References:
Comments: This probe contains amplified sequence from the first exon of Hoxd10.

Proper citation: RRID:Addgene_34712 Copy   

•   Source: Addgene

http://www.addgene.org/34833

Species: Caenorhabditis elegans
Genetic Insert: daf-16
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.

Proper citation: RRID:Addgene_34833 Copy   

•   Source: Addgene

http://www.addgene.org/34797

Species: Homo sapiens
Genetic Insert: PTD012
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.

Proper citation: RRID:Addgene_34797 Copy   

•   Source: Addgene

http://www.addgene.org/34792

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:Derived from pBluescript II SK+; Vector Types:Hydra Expression; Bacterial Resistance:Ampicillin
References:
Comments: https://www.ncbi.nlm.nih.gov/nuccore/85062532

Proper citation: RRID:Addgene_34792 Copy   

•   Source: Addgene

http://www.addgene.org/34837

Species: Homo sapiens
Genetic Insert: CD68 promoter/enhancer -680/+140
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The CD68 promoter/enhancer in this construct consists of ~680bp 5' of the ATG and the 83bp first intron of human CD68. Please note that Addgene's sequencing results found a single nucleotide deletion at bp# 440 when compared to the full plasmid sequence provided by the depositor. The depositing scientist states that it is probably a polymorphism in the promoter and is not a concern for the function of the plasmid.

Proper citation: RRID:Addgene_34837 Copy   

•   Source: Addgene

http://www.addgene.org/34836

Species: Mus musculus
Genetic Insert: GATA3
Vector Backbone Description: Backbone Marker:Hergen Spits and coworkers; Backbone Size:12443; Vector Backbone:LZRSpBMN-linker-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_34836 Copy   

•   Source: Addgene

http://www.addgene.org/34838

Species: E. Coli
Genetic Insert: LplA W37V
Vector Backbone Description: Backbone Marker:Gift from John Cronan; Backbone Size:4800; Vector Backbone:pYFJ16; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_34838 Copy   

•   Source: Addgene

http://www.addgene.org/34661

Species: Caenorhabditis elegans
Genetic Insert: slo-1(A2;B1;C1)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
References:
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.

Proper citation: RRID:Addgene_34661 Copy   

•   Source: Addgene

http://www.addgene.org/34701

Species: Rattus norvegicus
Genetic Insert: TGF beta type I receptor
Vector Backbone Description: Backbone Marker:Genentech; Backbone Size:4700; Vector Backbone:pRK5 modified with flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: 3 amino acids, GSM, were introduced after aa148 for cloning purposes.

Proper citation: RRID:Addgene_34701 Copy   

•   Source: Addgene

http://www.addgene.org/34821

Species: Homo sapiens
Genetic Insert: HORMA domain containing protein
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.

Proper citation: RRID:Addgene_34821 Copy   

•   Source: Addgene

http://www.addgene.org/34705

Species: Homo sapiens
Genetic Insert: TFAM
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_34705 Copy   

•   Source: Addgene

http://www.addgene.org/34826

Species: Homo sapiens
Genetic Insert: epithelial stromal interaction 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.

Proper citation: RRID:Addgene_34826 Copy   

•   Source: Addgene

http://www.addgene.org/34703

Species:
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Created by Köster and Fraser; Vector Backbone:act-GVP-UG; Vector Types:Xenopus expression; Bacterial Resistance:Kanamycin
References:
Comments: The Sox2/Oct3-4 enhancer elements and a minimal promoter from the murine FGF4 were subcloned from the plasmid described in Figure 1 of Ambrosetti et al. (2000). The fragment contains six tandem repeats of the 35-bp fragment containing the Sox2/Oct3-4 heterodimer transcription factor binding domain (bd) followed by a 165-bp minimal FGF4 promoter (mFGF4). This plasmid was a gift of Claudio Basilico (New York University). To increase the FP signal, the Sox2/Oct 3-4-mFGF regulatory fragment was moved to the “act-GVP-UG” concatenated plasmid created by Köster and Fraser (2001), which was adapted from the Drosophila Gal4-UAS system (Brand and Perrimon,1993) and shown to function in Xenopus (Chae et al.,2002; Hartley et al.,2002). We replaced the minimal promoters of the act-GVP-UG plasmid with our Sox2/Oct3-mFGF regulatory fragment to control the expression of the transcriptional activator, Gal4-VP16, which in turn drives the expression of genes controlled by the upstream activating sequence (UAS). The final expression vector (abbreviated as pSox2-bd::FP) contains: 6 repeats of the Sox2/Oct3-4 transcription factor binding domain, mFGF4, Gal4-VP16, a polyadenylation site, 14 repeats of UAS, mFGF4, and eGFP (ClonTech, Palo Alto, CA). Sequencing with EGFP-N identified 1nt deletion at position 1733, 1 nt insertion at position 1214, 2 nt insertion at 1557 compared to the depositor provided sequence. These mutations are all in non-coding regions and have been shown by the depositing lab to not effect the function of the plasmid.

Proper citation: RRID:Addgene_34703 Copy   

•   Source: Addgene