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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
bU6-sgMark2-mU6-sgMark3-hU6-sgMark4
 
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RRID:Addgene_177235 sgMark2/sgMark3/sgMark4 Mus musculus Ampicillin Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:09:05 0
bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2
 
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RRID:Addgene_177234 sgNeo1/sgNuak1/sgNuak2 Mus musculus Ampicillin Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:09:05 0
bU6-sgNeo1-mU6-sgNT-hU6-sgSnrk
 
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RRID:Addgene_177236 sgNeo1/sgNT1/sgSnrk Mus musculus Ampicillin Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:09:05 0
CJW4617
 
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RRID:Addgene_177118 MG1655 PlacZYA::Plac-gfp-muNS-kan Kanamycin PMID:24361104 GFP-muNS expression is under the control of Plac promoter integrated into the endogenous lac locus. The native lac operon (lacZYA) is deleted. Kanamycin resistance casette is inserted downstream of the gfp-muNS gene for cloning purposes. Cloning method: Lambda RED recombination. The gfp-μNS fragment was amplified from plasmid pER12 (pBAD322A-gfp-μNS), which encodes an N-terminal GFP fusion to residues 471-721 of μNS (Broering et al., 2005) and then digested by KpnI/XhoI. The nptI gene was amplified from pKD4 plasmid and digested by XhoI/SmaI. Then, gfp-μNS and nptI gene fragments were triple ligated into the pKS plasmid. The resulting plasmid was used as a PCR template to generate a gfp-μNS-nptI fragment with flanking regions complementary to lacI and cynX, respectively. This fragment was then used to replace the lac operon (lacZYA) by one-step λ-recombination (Datsenko and Wanner, 2000). The resulting gfp-μNS-nptI replacement of lacZYA was transduced back into MG1655 by P1-phage to generate strain CJW4617. See Parry et al., 2014 for more details. This strain doesn't contain any plasmid. Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin 2026-08-01 01:09:04 0
bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2
 
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RRID:Addgene_177231 sgNeo1/sgNT1/sgNeo2 E. coli Ampicillin Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:09:05 0
PB CAG-mTagBFP2-T2A-iCreERT2
 
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RRID:Addgene_177308 mTagBFP2-T2A-iCreERT2 Synthetic Ampicillin Optimized T2A sequence: https://doi.org/10.6084/m9.figshare.13636142.v79. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS) (#137864, https://www.addgene.org/137864/) was updated with (1) improved Kozak consensus sequence, (2) a slightly different codon usage in the 5' linker of the T2A, (3) a silently mutated XhoI site in iCre was reverted, and (4) four nucleotides were restored to the 5' of the WPRE. Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin 2026-08-01 01:09:05 0
pEDJ400
 
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RRID:Addgene_177269 Ampicillin PMID:34107026 Please note: Plasmid contains a C/T mixed base causing possible A50V mutation in URA3. This discrepancy is not known to affect plasmid function. Backbone Size:4320; Vector Backbone:p0054; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:09:05 0
pcDNA3 S6K2 KR
 
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1+ mentions
RRID:Addgene_17730 S6K2 Homo sapiens Ampicillin PMID:10490847 V390M is present, but should not affect protein function Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K99R: kinase dead mutant 2026-08-01 01:09:05 1
pcDNA3.1 mSTIM1 myc
 
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1+ mentions
RRID:Addgene_17732 STIM1 Mus musculus Ampicillin PMID:18327260 Mouse Stim1 (BglII/NotI, NotI is treated by Klenow) was subcloned into BamHI/XbaI (XbaI is treated by Klenow) of pcDNA3.1myc-His vector. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1 myc his; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:09:06 4
pENTR11 mSTIM1 myc
 
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RRID:Addgene_17733 STIM1 Mus musculus Kanamycin PMID:18327260 Mouse Stim1 (BglII/NotI) was subcloned into BglII/EagI followed by a myc tag. Backbone Size:2200; Vector Backbone:Modified pENTR11; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:09:06 0
pENTR11 mSTIM2 myc
 
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RRID:Addgene_17734 STIM2 Mus musculus Kanamycin PMID:18327260 Backbone Size:2200; Vector Backbone:Modified pENTR11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:09:06 0
pCLNR-H2BG
 
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1+ mentions
RRID:Addgene_17735 H2B-GFP Homo sapiens Ampicillin PMID:9545195 Backbone Size:7900; Vector Backbone:pCLNRX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:09:06 4
pOG231
 
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1+ mentions
RRID:Addgene_17736 NLS-Cre recombinase Ampicillin PMID:9405659 The Cre expression plasmid pOG231 was prepared by fusing a Cre coding sequence modified from pIC-Cre, and containing the same translation start and nuclear localization signal, to the synthetic intron and cytomegalovirus promoter of pOG44 Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS-; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-01 01:09:06 4
pX459-dEGF-exon1
 
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RRID:Addgene_177261 A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9 Other Ampicillin PMID:34667080 Backbone Marker:https://www.addgene.org/48139/; Backbone Size:3200; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-01 01:09:05 0
pLentiCRISPRv2-dADAM17 (#1)
 
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RRID:Addgene_177259 A gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9 Other Ampicillin PMID:34667080 Vector Backbone:LentiCRISPRv2puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-01 01:09:05 0
pQCXP CMV/TO V5-LUC (w476-1)
 
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RRID:Addgene_17740 Firefly Luciferase Ampicillin PMID:19657394 Backbone Size:7312; Vector Backbone:PQCXP; Vector Types:Mammalian Expression, Retroviral, Luciferase; Bacterial Resistance:Ampicillin 2026-08-01 01:09:06 0
pFastBac1 PIK3R1-Ni
 
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RRID:Addgene_17744 PIK3R1-Ni Homo sapiens Ampicillin PMID:15950905 Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Partial cDNA 2026-08-01 01:09:06 0
sTR060
 
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RRID:Addgene_177370 Trigger RNA Ampicillin PMID:35034449 Vector Backbone:Empty backbone; Vector Types:Synthetic Biology, Cell-Free Protein Synthesis; Bacterial Resistance:Ampicillin 2026-08-01 01:09:06 0
mCD8-GFP in pBS
 
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RRID:Addgene_17745 mouse CD8-GFP Mus musculus Ampicillin PMID:10197526 Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:09:06 0
pSSa23
 
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RRID:Addgene_177373 CgLUCopt-mut2 Other Ampicillin PMID:34682265 Vector Backbone:pVS20; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-01 01:09:06 0

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