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Species: Drosophila melanogaster
Genetic Insert: CG6701
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34518 Copy
Species: Drosophila melanogaster
Genetic Insert: CG5249
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34517 Copy
Species: Drosophila melanogaster
Genetic Insert: CG2714
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34516 Copy
Species: Drosophila melanogaster
Genetic Insert: CG9776
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34519 Copy
Species:
Genetic Insert: NLS-LSS-mKate2
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: There is an 18bp deletion in Addgene's sequencing results relative to the full plasmid sequence (at position 2174-2192bp). This region is immediately after the LSS-mKate2 gene and is not critical for plasmid performance.
There is also a single nucleotide mismatch that results in an F22S change in the linker between the NLS and LSS-mKate2. It should not have any effect on the fusion performance.
Two other mismatches do not alter amino acid sequence.
Proper citation: RRID:Addgene_34586 Copy
Species: Mus musculus
Genetic Insert: Slug
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3xFlag-CMV-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34584 Copy
Species:
Genetic Insert: tRNA^Sep
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_34624 Copy
Species: Methanocaldococcuc maripaludis
Genetic Insert: SepRS
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4300; Vector Backbone:pKK223-3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.
Certain elements of this plasmid are covered under US patent 9,090,928 to Yale University
Proper citation: RRID:Addgene_34623 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Morganstern JP and Land H 1990; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34589 Copy
Species: Mus musculus
Genetic Insert: Snail
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3xFlag-CMV-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34583 Copy
Species: Mus musculus
Genetic Insert: Arg1
Vector Backbone Description: Vector Backbone:pMIY; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34580 Copy
Species: Mus musculus
Genetic Insert: ASS1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1 hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34576 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34611 Copy
Species: Mus musculus
Genetic Insert: ASL
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1 hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34577 Copy
Species: Mus musculus
Genetic Insert: NFIL-3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1 hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34572 Copy
Species: Mus musculus
Genetic Insert: Arg1 promoter/enhancer -31/-3810
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34571 Copy
Species: Mus musculus
Genetic Insert: Arg1 base promoter -31/-2365
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34570 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:2070; Vector Backbone:pPROLar.A122; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: MG1655 for expression
Proper citation: RRID:Addgene_34617 Copy
Species:
Genetic Insert: synthetic TEAD luciferase reporter
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pGL3b; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Addgene sequencing detected a 7 nucleotide insertion at position 107 relative to the author's insert sequence. This results in the loss of a MscI restriction site but does not alter the function of the plasmid.
Please click http://www.addgene.org/34615/notes/ for general advice and technical tips on how to monitor YAP/TAZ regulation and activity in cell culture by 8xGTIIC lux.
Proper citation: RRID:Addgene_34615 Copy
Species:
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pMSCVhygro; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34565 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
