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Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18356 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18357 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18359 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10572187
Comments: See scanned map.
This is the original RET-EGFP vector. The highest virus titer that can be obtained with this construct is ~4x10^4 cfu/ml: the mRNA instability signal seems to block transcription of the virus genome at least to some extent. There are two versions, N1 and N2. Version N1 contains two XbaI sites and can be used for transfection after isolation of the XbaI-XbaI fragment. However, N1 contains an additional cryptic poly A signal between EGFP and GHpA, and its orientation is the same as that of virus transcription (from right to left in the figure). This means that N1 can not be used for the virus production: a full-length virus RNA is not produced efficiently. In contrast, N2 does not have such a cryptic poly A signal and is suitable for virus production. However, N2 contains an additional XbaI site between EGFP and GHpA, making it very difficult to isolate all the essential components of the vector on a single XbaI-XbaI fragment. Because of a weak NEO promoter, the number of G418-resistant colonies obtained after transfection (N1) or infection (N2) is fewer than that generated by other RET vectors with a strong NEO promoter. (Leder #C-1011)
Proper citation: RRID:Addgene_1834 Copy
Species: Arabidopsis thaliana
Genetic Insert: PYR1
Vector Backbone Description: Backbone Size:1774; Vector Backbone:pYTK084; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35726092
Proper citation: RRID:Addgene_183176 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10572187
Comments: See scanned map (#4).
The HSV-TK cassette is eliminated from this vector. Virus production should be fine, but you can not titrate the virus accurately because of the lack of the HSV-TK cassette, the only constitutive marker in RET vectors. You can easily excise all the essential components as an XbaI-XbaI fragment. To obtain larger numbers of G418-resistant clones, this vector carries a strong NEO promoter. This RET vector would be the first choice for making gene-disrupted mice by using transfected or infected ES cells. (Leder #C-1023)
Proper citation: RRID:Addgene_1836 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10572187
Comments: See scanned map (#5).
This is a modified RET vector exclusively for the DEL.BANK project (JC Schimenti, the Jackson Lab): the PGK promoter for the HSV-TK gene is much stronger than the MC1 promoter in other RET vectors. Virus production is virtually impossible. Either the PGK promoter or PGK poly A signal seems to be very inhibitory for the transcription of the virus genome. You can easily excise the essential components as an XbaI-XbaI fragment.
(Leder #C-1024)
Proper citation: RRID:Addgene_1837 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18320 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18322 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18323 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10572187
Comments: See scanned map.
This is the original RET construct for in vitro mutagenesis experiments. The highest virus titer that can be obtained with this construct is ~4x10^4 cfu/ml: the mRNA instability signal seems to block transcription of the virus genome at least to some extent. This construct can not be used for expresssion pattern analysis because of lack of IRES-EGFP. The XbaI-XbaI fragment can be used for transfection. Enhancer sequence in the U3 portion of the 5' LTR is not included in this XbaI-XbaI fragment.
(Leder #C-1021)
Proper citation: RRID:Addgene_1832 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18324 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10572187
Comments: See scanned map.
This is the original RET-EGFP vector. The highest virus titer that can be obtained with this construct is ~4x10^4 cfu/ml: the mRNA instability signal seems to block transcription of the virus genome at least to some extent. There are two versions, N1 and N2. Version N1 contains two XbaI sites and can be used for transfection after isolation of the XbaI-XbaI fragment. However, N1 contains an additional cryptic poly A signal between EGFP and GHpA, and its orientation is the same as that of virus transcription (from right to left in the figure). This means that N1 can not be used for the virus production: a full-length virus RNA is not produced efficiently. In contrast, N2 does not have such a cryptic poly A signal and is suitable for virus production. However, N2 contains an additional XbaI site between EGFP and GHpA, making it very difficult to isolate all the essential components of the vector on a single XbaI-XbaI fragment. Because of a weak NEO promoter, the number of G418-resistant colonies obtained after transfection (N1) or infection (N2) is fewer than that generated by other RET vectors with a strong NEO promoter.
(Leder #C-1010)
Proper citation: RRID:Addgene_1833 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18326 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18327 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18330 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18332 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18333 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18334 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18337 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
