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Species: Aequorea victoria, E. coli
Genetic Insert: Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
Vector Backbone Description: Backbone Size:1672; Vector Backbone:CA1; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Poser et al., Nature Meth. 5, (2008), 409-415
Proper citation: RRID:Addgene_28226 Copy
Species: SV40
Genetic Insert: SV40 large T
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10200; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28220 Copy
Species: Homo sapiens
Genetic Insert: ER beta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_28237 Copy
Species: Homo sapiens
Genetic Insert: Androgen receptor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Addgene's sequencing result indicates a loss of Q58 in the unstable poly-glutamine region. Our sequencing also shows that the BamHI site at the 3' end of the insert was destroyed. Please see our quality control sequence for the most accurate look at this region.
Tilley, W.D., Marcelli, M., Wilson, J.D. and McPhaul, J.M. (1989) Characterization and cloning of a cDNA encoding the human androgen receptor. Proc. Natl Acad. Sci. USA, 86, 327–331
Proper citation: RRID:Addgene_28235 Copy
Species: Homo sapiens
Genetic Insert: l(3)mbt-like 2 (Drosophila)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag4A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_28232 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor alpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Tzukerman MT, Esty A, Santiso-Mere D, Danielian P, Parker MG, Stein RB, Pike JW, McDonnell DP 1994 Human estrogen receptor transactivational capacity is determined by both cellular and promoter context and mediated by two functionally distinct intramolecular regions. Mol Endocrinol 8:21–30
Proper citation: RRID:Addgene_28230 Copy
Species: Homo sapiens
Genetic Insert: Tar DNA binding protein
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pCAG-EGFP/RFP-int; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28205 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Cellecta; Backbone Size:7500; Vector Backbone:pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28289 Copy
Species: Danio rerio
Genetic Insert: Flrt3 mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See map and Genbank file for plasmid details.
Proper citation: RRID:Addgene_28217 Copy
Species: Mus musculus
Genetic Insert: Oct4, Sox2, Klf4, c-Myc, Lin28
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:10200; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that Addgene's sequencing results using the CMV-F and pCEP-F primers found 2 sequence variants within and after the CMV enhancer. One is a C->G mismatch at position 775 within the CMV enhancer and the second is a deletion at position 949 just after the end of the CAG enhancer, as compared to the full plasmid sequence provided by the depositing scientist. The significance of the two sequence variants is unclear; however, the plasmid functions as described in the associated publication. Addgene's other 4 sequencing results completely matched the depositor's sequence.
Proper citation: RRID:Addgene_28213 Copy
Species: Mus musculus
Genetic Insert: NHERF1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV-2-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28297 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV-2-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28298 Copy
Species: Homo sapiens
Genetic Insert: NHERF1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV-2-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28295 Copy
Species: A. victoria
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5047; Vector Backbone:AAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox, Adeno-associated virus; Bacterial Resistance:Ampicillin
References:
Comments: PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.
Proper citation: RRID:Addgene_28304 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV-2-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28300 Copy
Species: Plasmodium berghei
Genetic Insert: PP-CDPK1
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7737; Vector Backbone:pET15-MHL (Addgene plasmid 26092); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: PDB: 3Q5I. http://www.thesgc.org/structures/3Q5I/
Proper citation: RRID:Addgene_28280 Copy
Species:
Genetic Insert: pCAGGS-mRFP
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28311 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIB1 (1-171aa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8000; Vector Backbone:pGADT7rec; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_28246 Copy
Species: Arabidopsis thaliana
Genetic Insert: cry2
Vector Backbone Description: Backbone Size:7480; Vector Backbone:na; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_28244 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIB1(deltaNLS)-pmGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_28240 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
