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Species: Synthetic
Genetic Insert: moxGFP-P2A-T2A
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:5857; Vector Backbone:pSBbi-Pur (Addgene Plasmid #60523); Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
References:
Comments: The moxGFP-P2A-T2A construct was designed by Jorge L. Arias-Arias from Universidad de Costa Rica ([email protected]). It was codon optimized for human and mouse expression and includes two additional cloning sites (5' EcoRV - 3' SalI and 5' SmaI - 3' XbaI, see the map uploaded by the depositor) for the polycistronic expression of up to three genes: moxGFP and two more. This vector is suitable for transient transfection, but it was intended to be co-transfected with the transposase vector pCMV(CAT)T7-SB100 (Addgene Plasmid #34879) in order to generate mammalian cells with stable expression of the genes of interest. Enjoy!
Proper citation: RRID:Addgene_176893 Copy
Species: Homo sapiens
Genetic Insert: Granulin 7
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176931 Copy
Species: Mus musculus
Genetic Insert: Integrin-linked kinase
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437490v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176897 Copy
Species: Homo sapiens
Genetic Insert: Granulin 7+linker8
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176930 Copy
Species: Homo sapiens
Genetic Insert: Granulin 3
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176923 Copy
Species: Homo sapiens
Genetic Insert: Granulin 4
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176925 Copy
Species: Homo sapiens
Genetic Insert: Granulin 6+linker7
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176928 Copy
Species: Homo sapiens
Genetic Insert: Granulin 5
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176927 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha S180A S204A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See article for cloning details. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17700 Copy
Species: Homo sapiens
Genetic Insert: APP
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: APP (EU832797.1) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176954 Copy
Species: Homo sapiens
Genetic Insert: RRP1
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: RRP1 (NM_003683) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176957 Copy
Species: Homo sapiens
Genetic Insert: RWDD2B
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: RWDD2B (NM_016940) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176956 Copy
Species: Homo sapiens
Genetic Insert: PCBP3
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: RRP1 (BC012061) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176958 Copy
Species: Homo sapiens
Genetic Insert: CRYZL1
Vector Backbone Description: Backbone Marker:Derived from Addgene #107398; KanR replaced with AmpR; Backbone Size:9341; Vector Backbone:plenti-CAG-gate-FLAG-IRES-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177004 Copy
Species: Homo sapiens
Genetic Insert: SLC19A1
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: SLC19A1 (BC003068) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176951 Copy
Species: Homo sapiens
Genetic Insert: B3GALT5
Vector Backbone Description: Backbone Marker:Derived from Addgene #107398; KanR replaced with AmpR; Backbone Size:9341; Vector Backbone:plenti-CAG-gate-FLAG-IRES-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177006 Copy
Species: Homo sapiens
Genetic Insert: FAM207A
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: FAM207A (NM_058190) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176950 Copy
Species: Homo sapiens
Genetic Insert: ETS2
Vector Backbone Description: Backbone Marker:Derived from Addgene #107398; KanR replaced with AmpR; Backbone Size:9341; Vector Backbone:plenti-CAG-gate-FLAG-IRES-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177005 Copy
Species: Homo sapiens
Genetic Insert: S100B
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: S100B (NM_006272) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176952 Copy
Species: Mus musculus
Genetic Insert: Rcan1
Vector Backbone Description: Backbone Marker:Derived from Addgene #107398; KanR replaced with AmpR; Backbone Size:9341; Vector Backbone:plenti-CAG-gate-FLAG-IRES-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177000 Copy
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