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Species: Homo sapiens
Genetic Insert: SFXN1
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: CCSB-DFCI hORFeome collection(31009_G02)
Proper citation: RRID:Addgene_111676 Copy
Species: Homo sapiens
Genetic Insert: TGON2
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: CCSB-DFCI hORFeome collection(11037_A04)
Proper citation: RRID:Addgene_111677 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111784 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111782 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111783 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid is for theophylline-inducible expression of APEX2 in the cytoplasm of mycobacteria. It is a single-copy integrating vector in mycobacteria. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111700 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111788 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid is for IPTG-inducible expression of APEX2 in the E. coli cytoplasm. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111702 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pGEMT-easy; Vector Types:cloning intermediate; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_111773 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111777 Copy
Species: Mus musculus
Genetic Insert: Mef2c, Tbx5 and Gata4
Vector Backbone Description: Backbone Size:5871; Vector Backbone:pMx; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111810 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111778 Copy
Species:
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pGEMT-easy; Vector Types:cloning intermediate; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_111812 Copy
Species:
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Marker:Cellbiolabs; Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
NheI right after iRFP670 was destroyed during cloning, containing half SpeI and half NheI.
Proper citation: RRID:Addgene_111816 Copy
Species: Homo sapiens
Genetic Insert: GGA2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11177 Copy
Species: Synthetic
Genetic Insert: Clover-GGSGGS9-mRuby2 fusion
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5402; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Partially based on Addgene Plasmids #49089 and #21769
Proper citation: RRID:Addgene_111762 Copy
Species: Synthetic
Genetic Insert: Clover-GGSGGS5-mRuby2 fusion
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5402; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Partially based on Addgene Plasmids #49089 and #21769
Proper citation: RRID:Addgene_111760 Copy
Species: Homo sapiens
Genetic Insert: RBAP2
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11174 Copy
Species: Homo sapiens
Genetic Insert: BLM
Vector Backbone Description: Backbone Marker:Invitrogen (thermoFisher); Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111766 Copy
Species: Gallus gallus
Genetic Insert: Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5399; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Partially based on Addgene Plasmids #26019, #49089, and #21769
Proper citation: RRID:Addgene_111764 Copy
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