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Species: Synthetic
Genetic Insert: TetR and xCas9
Vector Backbone Description: Vector Backbone:p15A origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_111656 Copy
Species: Homo sapiens
Genetic Insert: ARP2
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: CCSB-DFCI hORFeome collection(11035_E02)
Proper citation: RRID:Addgene_111658 Copy
Species: Synthetic
Genetic Insert: PTE_28
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6634; Vector Backbone:pMal C2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111641 Copy
Species: Synthetic
Genetic Insert: PTE_36
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6634; Vector Backbone:pMal C2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111642 Copy
Species: Synthetic
Genetic Insert: ACS_9
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:5367; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111647 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP82
Vector Backbone Description: Backbone Marker:ATCC (#77189); Backbone Size:4453; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_1116 Copy
Species: Homo sapiens
Genetic Insert: FLAG-hsDicer
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6264; Vector Backbone:pMSCV-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Cloning performed in 2 steps: 1) PCR amplification of the original plasmid using primers featuring an overhang containing an XhoI restriction site, 2) Digestion of the pMSCV puro plamid by XhoI and ligation of the insert
Proper citation: RRID:Addgene_111630 Copy
Species:
Genetic Insert: sgRNA
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111595 Copy
Species: Mus musculus
Genetic Insert: mU6-sgRNA
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS results identified a V163A variant in EGFP, which does not affect plasmid function.
Proper citation: RRID:Addgene_111596 Copy
Species: Synthetic
Genetic Insert: dPTE2
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6634; Vector Backbone:pMal C2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111634 Copy
Species: Synthetic
Genetic Insert: PTE_5
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6634; Vector Backbone:pMal C2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111635 Copy
Species: Synthetic
Genetic Insert: PTE_25
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6634; Vector Backbone:pMal C2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111638 Copy
Species: Synthetic
Genetic Insert: PA1/04/03-eyfp
Vector Backbone Description: Vector Backbone:pBK-miniTn7-omegaGm (pUC-based); Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_111620 Copy
Species: Mus musculus
Genetic Insert: H2Kb
Vector Backbone Description: Backbone Marker:Addgene # 60523; Backbone Size:5661; Vector Backbone:pSBbi-pur; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111623 Copy
Species:
Genetic Insert: Venus-LEQLE-SIINFEKL-TEW
Vector Backbone Description: Backbone Marker:Addgene # 26553; Vector Backbone:pT2/SV-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111624 Copy
Species:
Genetic Insert: yellow pre-eGRASP(p30)
Vector Backbone Description: Backbone Size:4375; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111588 Copy
Species:
Genetic Insert: cyan pre-eGRASP(p32)
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111589 Copy
Species: Mus musculus
Genetic Insert: Bax
Vector Backbone Description: Backbone Marker:Mark Van Delft; Backbone Size:7006; Vector Backbone:pMIH/MSCV-IRES-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111628 Copy
Species: Mus musculus
Genetic Insert: TOMM20
Vector Backbone Description: Backbone Marker:Mark Van Delft; Backbone Size:6969; Vector Backbone:pMIH/MSCV-IRES-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111626 Copy
Species: Homo sapiens
Genetic Insert: Exonuclease 1a
Vector Backbone Description: Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_111629 Copy
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