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Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments: The plasmid differs from the MAVS reference sequence at 2 positions (Q198K and S409F). This plamid has an additional Q271A mutation, and amino acid 93 (glutamate, E) was mutated back to the reference (glutamine, Q). Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q271A and a second mutagenesis to yield the double mutant. The clones displayed consistent off-target mutations at one of two points. Two clones with different off-target mutations were digested and the good pieces ligated together so to yield the final sequence.
Proper citation: RRID:Addgene_158632 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments: The plasmid differs from the MAVS reference sequence at 3 positions: Q93E, Q198K, and S409F. This plasmid has an additional Q148A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) on the pEN_TT 3XFLAG-MAVS plasmid.
Proper citation: RRID:Addgene_158630 Copy
Species: Homo sapiens
Genetic Insert: MAP7 (Microtubule Associated Protein 7)
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_159718 Copy
Species: Homo sapiens
Genetic Insert: KIF5B (only amino acids 1-560), K560
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_159720 Copy
Species: Homo sapiens
Genetic Insert: KIF5B (only amino acids 1-490), K490
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_159721 Copy
Species: Homo sapiens
Genetic Insert: MAP7 (only amino acids 1-316)(includes MTBD and P123 domains)
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_159728 Copy
Species: Homo sapiens
Genetic Insert: KIF5B (full length, 1-963 amino acids), K963
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_159727 Copy
Species:
Genetic Insert: yhaV toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_127455 Copy
Species:
Genetic Insert: tse2 toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_127457 Copy
Species: Arabidopsis thaliana
Genetic Insert: TESTA ABUNDANT 2
Vector Backbone Description: Backbone Marker:Invitrogen modified by doi:10.1271/bbb.70678; Backbone Size:5892; Vector Backbone:pDONRG_P4-P1R; Vector Types:Gateway promoter entry clone; Bacterial Resistance:Gentamicin
References:
Comments: For use with Three-way Multi-site Gateway Cloning (Invitrogen).
Proper citation: RRID:Addgene_128429 Copy
Species: P. aeruginosa
Genetic Insert: PapRecT
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSEVA258; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_128971 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Esteban Martínez-García and Víctor de Lorenzo; Backbone Size:3168; Vector Backbone:pEMG; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_122093 Copy
Species:
Genetic Insert: B. subtilis Rex
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_122134 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_160206 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: E
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments: Kim et al. 2020 can be accessed at https://doi.org/10.1016/j.cell.2020.04.011
Proper citation: RRID:Addgene_153955 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway-compatible Entry vector, with mutagenized insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_154029 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP3
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR207; Vector Types:Gateway-compatible Entry vector, with insert of NSP3 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_154402 Copy
Species: Homo sapiens
Genetic Insert: DYNLRB1
Vector Backbone Description: Backbone Marker:Geneva Biotech; Backbone Size:2922; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments: Cloned by Ruta Zalyte in Andrew Carter's lab. For cloning strategy, please refer to the Methods section of Toropova et al 2019.
Proper citation: RRID:Addgene_132533 Copy
Species: Homo sapiens
Genetic Insert: DYNLT3
Vector Backbone Description: Backbone Marker:Geneva Biotech; Backbone Size:2922; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments: Cloned by Ruta Zalyte in Andrew Carter's lab. For cloning strategy, please refer to the Methods section of Toropova et al 2019.
Proper citation: RRID:Addgene_132532 Copy
Species: Homo sapiens
Genetic Insert: DYNLT1
Vector Backbone Description: Backbone Marker:Geneva Biotech; Backbone Size:2924; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments: Cloned by Ruta Zalyte in Andrew Carter's lab. For cloning strategy, please refer to the Methods section of Toropova et al 2019.
Proper citation: RRID:Addgene_132528 Copy
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