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URL: http://rgd.mcw.edu/tools/strains/strains_view.cgi?id=152999023
Proper Citation: RRID:RRRC_00965
Description: Rattus norvegicus with name SD-Aif1tm(EGFP)Apps/Mmmc from RGD.
Species: Rattus norvegicus
Notes: Applied StemCell, Inc (Milpitas, CA) was contracted to generate the Iba1-EGFP knock-in rat model using CRISPR/Cas9 technology in the Sprague Dawley rat strain. The donor construct inserted consisted of the EGFP coding sequence (minus the first ATG), followed by the 22 amino acid sequence of the porcine teschovirus-1 2A (P2A) self-cleaving peptide, and then the first exon of the rat Iba1 gene immediately downstream of the translational start site. Guide RNA with the following sequence: 5'- TACCCTGCAAATCCTTGCTCTGG-3' targeting the Iba1 gene just downstream of the translational start site were used. Rat Resource and Research Center
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Source: Integrated Animals
Source Database: Rat Resource and Research Center (RRRC)
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
