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URL: http://www.addgene.org/41551
Proper Citation: RRID:Addgene_41551
Insert Name: TRAM
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:14517278
Vector Backbone Description: Backbone Marker:Mizushima and Nagata (Osaka Bioscience Institute, Japan, 1990) (PMID: 1698283) ; Backbone Size:5685; Vector Backbone:pEF-Bos; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The depositing lab generated pEF-Bos Tram Flag from a human peripheral blood mononuclear cell (PBMC) complementary DNA library by PCR amplification and cloning. It was cloned with XhoI at the 5' end and BamHI at the 3' end. NotI can be used at the 3' end to excise the tagged CDS. The construct also contains a HIS tag.
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
