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URL: http://www.addgene.org/117126
Proper Citation: RRID:Addgene_117126
Insert Name: 6xHA Tag
Bacterial Resistance: Ampicillin
Defining Citation: PMID:31147909
Vector Backbone Description: Backbone Marker:Thermo scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Golden gate donor vector for gene targeting in Bacillus subtilis; Bacterial Resistance:Ampicillin
Comments: Donor vector for Golden gate assembly reactions that encodes for a C-terminal 6xHA tag. Overhangs generated upon BsaI cleavage : AGCG (RtagN) and TAAG (RtagC). (Gruber lab reference pSG1106) Addgene NGS results identified an IS1 element located between the CAP binding site and the lac UV5 promoter. This element will not affect plasmid function.
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
