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URL: http://www.addgene.org/117117
Proper Citation: RRID:Addgene_117117
Bacterial Resistance: Kanamycin
Defining Citation: PMID:31147909
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pET-Gate2; Vector Types:Backbone for Golden gate assembly for gene targeting in Bacillus subtilis; Bacterial Resistance:Kanamycin
Comments: Backbone vector for reconstitution of gene targeting constructs by Golden gate assembly. The MazEF toxin-antitoxin system couter-selects for single cross-over events that leads to incorporation of the vector backbone into the genome. Overhangs generated upon BsaI cleavage : CGAG (RU) and CCAT (RD). (Gruber lab reference pSG1525)
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
