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URL: http://www.addgene.org/65173
Proper Citation: RRID:Addgene_65173
Insert Name: tTDH1
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:25871405
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Type 4b parts are terminators used when a type 4a has been included as a coding sequence. For more information, please visit the supplemental information in PMID:25871405. This plasmid stock has been found to contain dimers. Dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
