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URL: http://www.addgene.org/49947
Proper Citation: RRID:Addgene_49947
Insert Name: 3x FLAG Tet1CDmut RH-4
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24108092
Vector Backbone Description: Backbone Marker:Joung lab (Addgene plasmid #49237); Vector Backbone:pJFA334E9; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Comments: TALE target site is: TCAGCACAGCAGCTTCCCCC. Note that due to the TET1 mutations, this plasmid neither demethylated the proximal CpGs nor activated RHOXF2 gene expression. See Supplementary Figure 13 of associated publication for amino acid sequences of backbone and TALE repeat array.
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Source: Addgene