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URL: http://www.addgene.org/45061
Proper Citation: RRID:Addgene_45061
Insert Name: NgCam
Organism: Gallus gallus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10839364
Vector Backbone Description: Backbone Size:6755; Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NgCam is very GC rich and difficult to sequence and PCR. pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination. XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains. Addgene's sequencing results found a few nucleotide differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositor, these differences are not a concern for the function of the plasmid.
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
