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URL: http://www.addgene.org/34928
Proper Citation: RRID:Addgene_34928
Bacterial Resistance: None
Defining Citation: PMID:21868676
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:None
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain Top10. Markerless gene deletions were carried out using a λ-red and FLP recombinase-based gene knockout strategy.
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
