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URL: http://www.addgene.org/34623
Proper Citation: RRID:Addgene_34623
Insert Name: SepRS
Organism: Methanocaldococcuc maripaludis
Bacterial Resistance: Kanamycin
Defining Citation: PMID:21868676
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4300; Vector Backbone:pKK223-3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid. Certain elements of this plasmid are covered under US patent 9,090,928 to Yale University
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
