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URL: http://www.addgene.org/33359
Proper Citation: RRID:Addgene_33359
Insert Name: TetR cassette
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pCRII; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Comments: Do not select for this plasmid with tetracycline, as we have found that the Tet resistance cassette does not work reliably in high-copy plasmids such as this one. The Tet cassette MUST be transferred to a low-copy vector, e.g. a BAC, to allow Tet resistance in a reliable fashion. In BAC clones the Tet cassette has been extremely reliable.
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
